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Potential forensic application of DNA methylation profiling to body fluid identification.

Authors
 Hwan Young Lee  ;  Myung Jin Park  ;  Ajin Choi  ;  Ja Hyun An  ;  Woo Ick Yang  ;  Kyoung-Jin Shin 
Citation
 INTERNATIONAL JOURNAL OF LEGAL MEDICINE, Vol.126(1) : 55-62, 2012 
Journal Title
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
ISSN
 0937-9827 
Issue Date
2012
MeSH
Adaptor Proteins, Signal Transducing/genetics ; Blood ; Body Fluids* ; Crime ; DNA Methylation* ; Epigenesis, Genetic ; Female ; Forensic Genetics* ; Gene Expression Profiling* ; Homeodomain Proteins/genetics ; Humans ; Intracellular Signaling Peptides and Proteins/genetics ; Male ; Nuclear Proteins/genetics ; Profilins/genetics ; Protein-Arginine N-Methyltransferases/genetics ; Saliva ; Semen ; Ubiquitin Thiolesterase/genetics ; Vagina/secretion
Keywords
Body fluid identification ; DNA methylation ; Tissue-specific differentially methylated region
Abstract
DNA analysis of various body fluid stains at crime scenes facilitates the identification of individuals but does not currently determine the type and origin of the biological material. Recent advances in whole genome epigenetic analysis indicate that chromosome pieces called tDMRs (tissue-specific differentially methylated regions) show different DNA methylation profiles according to the type of cell or tissue. We examined the potential of tissue-specific differential DNA methylation for body fluid identification. Five tDMRs for the genes DACT1, USP49, HOXA4, PFN3, and PRMT2 were selected, and DNA methylation profiles for these tDMRs were produced by bisulfite sequencing using pooled DNA from blood, saliva, semen, menstrual blood, and vaginal fluid. The tDMRs for DACT1 and USP49 showed semen-specific hypomethylation, and the tDMRs for HOXA4, PFN3, and PRMT2 displayed varying degrees of methylation according to the type of body fluid. Preliminary tests using methylation-specific PCR for the DACT1 and USP49 tDMRs showed that these two markers could be used successfully to identify semen samples including sperm cells. Body fluid-specific differential DNA methylation may be a promising indicator for body fluid identification. Because DNA methylation profiling uses the same biological source of DNA for individual identification profiling, the determination of more body fluid-specific tDMRs and the development of convenient tDMR analysis methods will facilitate the broad implementation of body fluid identification in forensic casework.
Full Text
http://link.springer.com/article/10.1007%2Fs00414-011-0569-2
DOI
21626087
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Shin, Kyoung Jin(신경진) ORCID logo https://orcid.org/0000-0002-1059-9665
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Lee, Hwan Young(이환영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90484
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