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Prognostic impact of the cancer stem cell-related marker NANOG in ovarian serous carcinoma.

Authors
 Lee, Maria ; Nam, Eun Ji ; Kim, Young Tae ; Kim, Jae Hoon ; Kim, Sunghoon ; Kim, Sang Wun 
Citation
 International Journal of Gynecological Cancer, Vol.22(9) : 1489~1496, 2012 
Journal Title
 International Journal of Gynecological Cancer 
ISSN
 1048-891X 
Issue Date
2012
Abstract
OBJECTIVE: The objective of this study was to evaluate the prognostic significance of NANOG expression in ovarian serous carcinoma. METHODS: The expression of NANOG was evaluated in 6 ovarian carcinoma cell lines, paclitaxel-resistant SKOV3 cells, and SKOV3 spheroid cells with semiquantitative reverse transcription-polymerase chain reaction and Western blotting. NANOG expression was also measured immunohistochemically in a tissue microarray containing ovarian tissues from 74 patients with ovarian serous carcinoma and 24 with ovarian serous cystadenoma. Each sample was scored based on signal intensity and proportion, and a score greater than 4 was considered "positive." RESULTS: NANOG mRNA expression was variable in different ovarian cancer cell lines. The mRNA level of NANOG was increased in the paclitaxel-resistant SKOV3 cells and SKOV3 spheroid cells compared with that in the SKOV3 cells. NANOG expression was positive in 21.6% of 74 ovarian serous carcinoma tissues, but none of the ovarian serous cystadenoma tissues were positive. Positive NANOG expression was associated with residual tumor size after surgery (P = 0.032). The overall survival of the patients with positive NANOG expression was poorer than that of the patients with negative NANOG expression (P = 0.020). In patients with stage I and II disease, positive NANOG expression was independently associated with shorter overall survival compared with negative NANOG expression (40 vs 120 months, respectively; P = 0.031). CONCLUSIONS: Positive NANOG expression is associated with poor prognosis of ovarian serous carcinoma. NANOG has potential as a predictor of survival for patients with ovarian carcinomas and may be involved in the mechanism of chemoresistance.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/90474
DOI
10.1097/IGJ.0b013e3182738307
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Obstetrics & Gynecology
Yonsei Authors
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Link
 http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00009577-201211000-00008&LSLINK=80&D=ovft
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