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The effect of sRAGE-Fc fusion protein attenuates inflammation and decreases mortality in a murine cecal ligation and puncture model.

 Su Jin Jeong  ;  Beom Jin Lim  ;  Sungha Park  ;  Donghoon Choi  ;  Hye Won Kim  ;  Nam Su Ku  ;  Sang Hoon Han  ;  Chang Oh Kim  ;  Jun Yong Choi  ;  Young Goo Song  ;  June Myung Kim 
 INFLAMMATION RESEARCH, Vol.61(11) : 1211-1218, 2012 
Journal Title
Issue Date
Animals ; Anti-Inflammatory Agents/therapeutic use ; Cecum/injuries ; Cytokines/genetics ; Disease Models, Animal ; Ligation ; Mice ; Mice, Inbred C57BL ; Pneumonia/drug therapy* ; Pneumonia/metabolism ; Pneumonia/pathology ; RNA, Messenger/metabolism ; Receptor for Advanced Glycation End Products ; Receptors, Immunologic/genetics ; Receptors, Immunologic/therapeutic use* ; Recombinant Fusion Proteins/therapeutic use* ; Sepsis/drug therapy* ; Sepsis/metabolism ; Sepsis/pathology
sRAGE-Fc fusion protein ; Severe sepsis ; Cecal ligation and puncture ; Mortality ; Cytokine
OBJECTIVE: Inhibition of the receptor for advanced glycation end products (RAGE) may attenuate the systemic inflammatory response and ensuing severe sepsis. We report an investigation into the effect of soluble RAGE (sRAGE)-Fc fusion protein in severe sepsis induced by a cecal ligation and puncture (CLP) procedure.

MATERIALS AND METHODS: The experiment was performed using CLP control mice, mice treated with 0.5 or 1.0 μg sRAGE-Fc fusion protein, and sham surgery mice.

RESULTS: Survival benefits over the CLP control group were evident (P = 0.036) in mice given 1.0 μg sRAGE-Fc fusion protein. In addition, the pulmonary inflammation score in the sRAGE-Fc fusion protein-treated group was significantly lower than that in the CLP control group (P < 0.05). Lung tissue in the sRAGE-Fc fusion protein-treated group revealed a significant decrease in the expression of inflammatory cytokines. Furthermore, levels of interleukin (IL)-1β and tumor necrosis factor-α were significantly lower in sRAGE-Fc fusion protein treated groups (P < 0.001). Moreover, IL-6 levels showed a significant difference between CLP control and sRAGE-Fc fusion protein treated groups (P < 0.01).

CONCLUSIONS: sRAGE-Fc fusion protein has beneficial effects in a standard murine model of polymicrobial, intra-abdominal severe sepsis.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Ku, Nam Su(구남수) ORCID logo https://orcid.org/0000-0002-9717-4327
Kim, June Myung(김준명)
Kim, Chang Oh(김창오) ORCID logo https://orcid.org/0000-0002-0773-5443
Kim, Hye Won(김혜원)
Park, Sung Ha(박성하) ORCID logo https://orcid.org/0000-0001-5362-478X
Song, Young Goo(송영구) ORCID logo https://orcid.org/0000-0002-0733-4156
Lim, Beom Jin(임범진) ORCID logo https://orcid.org/0000-0003-2856-0133
Jeong, Su Jin(정수진) ORCID logo https://orcid.org/0000-0003-4025-4542
Choi, Dong Hoon(최동훈) ORCID logo https://orcid.org/0000-0002-2009-9760
Choi, Jun Yong(최준용) ORCID logo https://orcid.org/0000-0002-2775-3315
Han, Sang Hoon(한상훈) ORCID logo https://orcid.org/0000-0002-4278-5198
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