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Urinary vitamin D-binding protein is elevated in patients with endometriosis.

Authors
 SiHyun Cho  ;  Young Sik Choi  ;  Su Youn Yim  ;  Hyo In Yang  ;  Young Eun Jeon  ;  Kyung Eun Lee  ;  HyeYeon Kim  ;  Seok Kyo Seo  ;  Byung Seok Lee 
Citation
 HUMAN REPRODUCTION, Vol.27(2) : 515-522, 2012 
Journal Title
 HUMAN REPRODUCTION 
ISSN
 0268-1161 
Issue Date
2012
MeSH
Adult ; Biomarkers/urine ; Biomarkers, Tumor/urine ; DNA-Binding Proteins/urine ; Endometriosis/urine* ; Female ; Humans ; Middle Aged ; Phosphopyruvate Hydratase/urine ; Prealbumin/urine ; Protein Disulfide-Isomerases/urine ; Protein Subunits/urine ; Sensitivity and Specificity ; Tumor Suppressor Proteins/urine ; Up-Regulation* ; Vitamin D-Binding Protein/urine* ; Young Adult ; alpha 1-Antitrypsin/urine
Keywords
biomarker ; endometriosis ; proteomics ; urine ; vitamin D-binding protein
Abstract
BACKGROUND: Recently, proteomic technologies have demonstrated that several proteins are differently expressed in various body fluids of patients with endometriosis compared with those without this condition. The aim of this study was to investigate proteins secreted in urine of patients with endometriosis using proteomic techniques in order to identify potential markers for the clinical diagnosis of endometriosis. METHODS: Urine samples were collected from women undergoing laparoscopy for different indications including pelvic masses, pelvic pain, suspicious endometriosis, infertility and diagnostic evaluation. Proteomic techniques and mass spectrometry were used to identify proteins secreted in the urine of the patients with and without endometriosis and quantification of identified protein was performed using western blot and specific commercial sandwich enzyme-linked immunosorbent assays (ELISA). RESULTS: Twenty-two protein spots were differentially expressed in the urine of patients with and without endometriosis, one of which was identified as urinary vitamin D-binding protein (VDBP). ELISA quantification of urinary VDBP corrected for creatinine expression (VDBP-Cr) revealed that urinary VDBP-Cr was significantly greater in patients with endometriosis than in those without (111.96 ± 74.59 versus 69.90 ± 43.76 ng/mg Cr, P = 0.001). VDBP-Cr had limited value as a diagnostic marker for endometriosis (Sensitivity 58%, Specificity 76%). When combined with serum CA-125 levels (the product of serum CA-125 and urinary VDBP-Cr), it did not significantly increase the diagnostic power of serum CA-125 alone. CONCLUSIONS: Urinary VDBP levels are elevated in patients with endometriosis. They have limited value as a potential diagnostic biomarker for endometriosis but suggest it would be worthwhile to investigate other urinary proteins for this purpose.
Files in This Item:
T201200521.pdf Download
DOI
22158084
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Hye Yeon(김혜연)
Seo, Seok Kyo(서석교) ORCID logo https://orcid.org/0000-0003-3404-0484
Yang, Hyo In(양효인)
Lee, Kyung Eun(이경은)
Lee, Byung Seok(이병석) ORCID logo https://orcid.org/0000-0001-6001-2079
Yim, Su Youn(임수연)
Jeon, Young Eun(전영은)
Cho, Si Hyun(조시현) ORCID logo https://orcid.org/0000-0003-2718-6645
Choi, Young Sik(최영식) ORCID logo https://orcid.org/0000-0002-1157-4822
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90379
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