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Epithelial Na+ channel proteins are mechanotransducers of myogenic constriction in rat posterior cerebral arteries.

Authors
 Eok-Cheon Kim  ;  Duck-Sun Ahn  ;  Soo-In Yeon  ;  Mihwa Lim  ;  Young-Ho Lee 
Citation
 EXPERIMENTAL PHYSIOLOGY, Vol.97(4) : 544-555, 2012 
Journal Title
EXPERIMENTAL PHYSIOLOGY
ISSN
 0958-0670 
Issue Date
2012
MeSH
Animals ; Epithelial Sodium Channels/physiology* ; Male ; Mechanotransduction, Cellular/physiology* ; Muscle, Smooth, Vascular/physiology* ; Posterior Cerebral Artery/physiology* ; RNA, Small Interfering/genetics ; Rats ; Rats, Sprague-Dawley ; Vasoconstriction/physiology*
Keywords
Animals ; Epithelial Sodium Channels/physiology* ; Male ; Mechanotransduction, Cellular/physiology* ; Muscle, Smooth, Vascular/physiology* ; Posterior Cerebral Artery/physiology* ; RNA, Small Interfering/genetics ; Rats ; Rats, Sprague-Dawley ; Vasoconstriction/physiology*
Abstract
It has been suggested that mechanosensitive ion channels initiate myogenic responses in vessels; however, the molecular identity of the mechanosensitive ion channel complex is unknown. Although previous reports have suggested that epithelial Na(+) channel (ENaC) proteins are mechanotransducers in arteries, experimental evidence demonstrating that ENaC proteins are mechanotransducers are not fully elucidated. The goal of the present study was to determine whether the ENaC is a mechanotransducer for the myogenic response by providing supporting evidence in the rat posterior cerebral artery (PCA). We measured the effect of ENaC inhibition on the pressure-induced myogenic response, Ca(2+) concentration and 20 kDa myosin light chain (MLC(20)) phosphorylation. We detected expression of βENaC and γENaC subunits in rat PCA by Western blots and immunofluorescence. Inhibition of ENaCs with amiloride, ethyl isopropyl amiloride or benzamil blocked the myogenic response. Moreover, the myogenic response was inhibited in rat PCA transfected with βENaC and γENaC small interfering RNA. The myogenic response was inhibited by elimination of external Na(+), which was replaced with N-methyl-d-glucamine. Amiloride and nifedipine inhibited the pressure-induced increase in Ca(2+) concentration. Finally, MLC(20) increased when the intraluminal pressure was raised, and the pressure-induced increase in MLC(20) phosphorylation was inhibited by pretreatment with amiloride, and in arteries transfected with βENaC or γENaC small interfering RNA. Our results suggest that ENaCs may play an important role as mechanosensitive ion channels initiating pressure-induced myogenic responses in rat PCA.
Files in This Item:
T201201084.pdf Download
DOI
22090066
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eok Cheon(김억천)
Ahn, Duk Sun(안덕선) ORCID logo https://orcid.org/0000-0001-9351-6951
Yeon, Soo In(연수인)
Lee, Young Ho(이영호) ORCID logo https://orcid.org/0000-0002-5749-1045
Lim, Mi Hwa(임미화)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90282
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