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Sequence polymorphisms of Der f 1, Der p 1, Der f 2 and Der p 2 from Korean house dust mite isolates.

Authors
 Kyoung Yong Jeong  ;  In-Yong Lee  ;  Tai-Soon Yong  ;  Jae-Hyun Lee  ;  Eun-Jin Kim  ;  Joo-Shil Lee  ;  Chein-Soo Hong  ;  Jung-Won Park 
Citation
 EXPERIMENTAL AND APPLIED ACAROLOGY, Vol.58(1) : 35-42, 2012 
Journal Title
 EXPERIMENTAL AND APPLIED ACAROLOGY 
ISSN
 0168-8162 
Issue Date
2012
MeSH
Amino Acid Sequence ; Animals ; Antigens, Dermatophagoides/drug effects ; Antigens, Dermatophagoides/genetics* ; Antigens, Dermatophagoides/metabolism* ; Arthropod Proteins/drug effects ; Arthropod Proteins/genetics ; Arthropod Proteins/metabolism ; Cysteine Endopeptidases/drug effects ; Cysteine Endopeptidases/genetics ; Cysteine Endopeptidases/metabolism ; Dermatophagoides farinae/metabolism* ; Dermatophagoides pteronyssinus/metabolism* ; Molecular Sequence Data ; Polymorphism, Genetic* ; Republic of Korea
Keywords
Allergens ; Der f 1 ; Der f 2 ; Der p 1 ; Der p 2 ; Polymorphism
Abstract
Amino acid sequence variations have possible influences on the allergenicity of allergens and may be important factors in allergen standardization. This study was undertaken to investigate the sequence polymorphisms of group 1 and 2 allergens from Korean isolates of the house dust mites Dermatophagoides farinae and D. pteronyssinus. cDNA sequences encoding group 1 and 2 allergens were amplified by RT-PCR and compared the deduced amino acid sequences. Der f 1.0101, which appeared in 64.0 % of the 50 sequences analyzed, was found to be predominant. Among the Der p 1 sequences, Der p 1.0102 and 1.0105 were predominant (58 %). Among the Der f 2 sequences, Der f 2.0102 (40.7 %) and a new variant with Gly at position 42 (27.8 %) were predominant. The deduced amino acid sequences of 60 Der p 2 clones were examined, and 28 variants with 1-5 amino acid substitutions were found. Interestingly, all of the Der p 2 sequences had Thr instead of Lys at position 49. Two variants (Leu40, Thr49, and Asn114 (26.6 %); Val40, Thr49, and Asn114 (20.0 %)) were found to be the most predominant forms of Der p 2. Der p 1 has a high rate of sporadic substitutions and the group 2 allergens show a more regular pattern with orderly associations of amino acid substitutions. Der f 1 and Der p 2 from Korean mite isolates have unique amino acid sequence polymorphisms. These findings provide important data for house dust mite allergen standardization.
Full Text
http://link.springer.com/article/10.1007%2Fs10493-012-9553-x
DOI
22535483
Appears in Collections:
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Lee, In Yong(이인용)
Lee, Jae Hyun(이재현) ORCID logo https://orcid.org/0000-0002-0760-0071
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90259
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