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Effect of retinoic acid on epithelial differentiation and mucin expression in primary human corneal limbal epithelial cells.

 Sun Woong Kim  ;  Kyoung Yul Seo  ;  Taiyoun Rhim  ;  Eung Kweon Kim 
 CURRENT EYE RESEARCH, Vol.37(1) : 33-42, 2012 
Journal Title
Issue Date
Blotting, Western ; Cell Differentiation/drug effects* ; Cells, Cultured ; Epithelium, Corneal/drug effects ; Epithelium, Corneal/metabolism ; Epithelium, Corneal/ultrastructure* ; Gene Expression Regulation/drug effects* ; Humans ; Immunohistochemistry ; Keratolytic Agents/pharmacology ; Limbus Corneae/drug effects* ; Limbus Corneae/metabolism ; Limbus Corneae/ultrastructure ; Microscopy, Electron, Scanning Transmission ; Mucins/biosynthesis* ; Mucins/genetics ; RNA/genetics* ; Reverse Transcriptase Polymerase Chain Reaction ; Tretinoin/pharmacology*
Cell culture ; Corneal epithelial cell ; Limbal epithelium ; Mucin ; Retinoic acid
PURPOSE: Retinoic acid (RA) is essential for epithelial differentiation and maintenance of the mucous phenotype. This study investigated the effect of RA on corneal epithelial differentiation and mucin expression in a primary human corneal limbal epithelial cell (HCLEC) culture model.

METHODS: HCLECs were grown in RA-supplemented media at various concentrations (0, 10(-9) to 10(-6) M). Stratified HCLECs were examined using immunohistochemical or immunofluorescent staining for p63, ABCG2, CK3, CK19, and Western blotting for ABCG2 and CK12 to assess differentiation. Ultrastructural morphology was investigated using scanning and transmission electron microscopy. They were incubated with rose bengal dye to examine barrier function. The effects of RA on the expression of MUC1, -4, and -16 were analyzed by immunohistochemistry, quantitative real-time PCR and Western blot analysis.

RESULTS: HCLEC grown without RA showed hyperkeratosis, whereas those grown with 10(-8) to 10(-7) M RA induced non-keratinized stratified epithelium with a normal appearance. Under these conditions, p63, ABCG2, CK3, CK19, MUC1, -4, and -16 staining patterns were similar to in vivo limbal epithelium. A higher concentration (10(-6) M) of RA resulted in abnormal differentiation. HCLECs grown with RA were tightly apposed and maintained intact barrier function against dye penetration. In addition, MUC1, -4, and -16 expressions were highly associated with RA concentrations.

CONCLUSIONS: This study showed that cultured HCLEC could mimic physiologic and functional phenotypes by controlling RA concentrations in medium. Also, our results suggested modulating effect of RA on differentiation and mucin expression in corneal epithelium.
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1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sun Woong(김선웅)
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
Seo, Kyoung Yul(서경률) ORCID logo https://orcid.org/0000-0002-9855-1980
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