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Evaluation of revisited fucosylated alpha-fetoprotein (AFP-L3) with an autoanalyzer μTAS in a clinical laboratory.

Authors
 Jonghyeon Choi  ;  Yongjung Park  ;  Jeong-Ho Kim  ;  Hyon-Suk Kim 
Citation
 CLINICA CHIMICA ACTA, Vol.413(1-2) : 170-174, 2012 
Journal Title
 CLINICA CHIMICA ACTA 
ISSN
 0009-8981 
Issue Date
2012
MeSH
Aged ; Female ; Fucose/metabolism* ; Humans ; Laboratories/organization & administration* ; Male ; Middle Aged ; Reproducibility of Results ; alpha-Fetoproteins/metabolism*
Keywords
Alpha-fetoprotein (AFP) ; AFP-L3 ; Lens culinaris agglutinin (LCA) ; Liquid-phase binding assay
Abstract
BACKGROUND: We assessed clinical and analytical performances of AFP and fucosylated AFP (AFP-L3) assays by a newly developed automated analyzer based on liquid-phase binding (micro-total analysis systems, μTAS). METHODS: A total of 239 serum samples were obtained from 120 patients with hepatocellular carcinoma (HCC) and 119 without HCC. Precision of assays by the μTAS was evaluated, and the correlation between AFP-L3 and AFP levels was analyzed. Receiver operating characteristics curve-area under the curve (ROC-AUC) value was calculated to measure the diagnostic performance. RESULTS: Imprecision for AFP (ng/ml), AFP-L3 (ng/ml), and AFP-L3 (%) with 2 levels of QC materials was all within 5% coefficient of variation. AFP levels measured by the μTAS were correlated well with those by the UniCel DxI 800 Access (r=0.83). AFP-L3 concentrations in HCC patients were higher than those in control group (median 379.2 ng/ml in HCC, 1.0 ng/ml in non-HCC, P<0.05). AUC of AFP-L3 was 0.91 which was significantly higher than that of AFP (0.88 by μTAS; 0.84 by UniCel DxI 800, P<0.05 for both). CONCLUSION: AFP-L3 in HCC was significantly higher than that of control group. The μTAS showed good performances for routine uses in clinical laboratories for measuring AFP and AFP-L3.
Full Text
http://www.sciencedirect.com/science/article/pii/S0009898111005018
DOI
21946051
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jeong Ho(김정호) ORCID logo https://orcid.org/0000-0003-2479-0548
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Yong Jung(박용정)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89970
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