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Anti-inflammatory effect of quercetin in a whole orbital tissue culture of Graves' orbitopathy.

Authors
 Jin Sook Yoon  ;  Min Kyung Chae  ;  Sang Yeul Lee  ;  Eun Jig Lee 
Citation
 BRITISH JOURNAL OF OPHTHALMOLOGY, Vol.96(8) : 1117-1121, 2012 
Journal Title
BRITISH JOURNAL OF OPHTHALMOLOGY
ISSN
 0007-1161 
Issue Date
2012
MeSH
Adipose Tissue/drug effects* ; Adipose Tissue/metabolism ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology* ; Antioxidants/pharmacology* ; Connective Tissue/drug effects* ; Connective Tissue/metabolism ; Cytokines/genetics ; Cytokines/metabolism ; Enzyme-Linked Immunosorbent Assay ; Graves Ophthalmopathy/metabolism* ; Graves Ophthalmopathy/pathology ; Humans ; L-Lactate Dehydrogenase/metabolism ; Orbital Diseases/metabolism* ; Orbital Diseases/pathology ; Organ Culture Techniques ; Quercetin/pharmacology* ; RNA, Messenger/metabolism ; Real-Time Polymerase Chain Reaction
Keywords
Adipose Tissue/drug effects* ; Adipose Tissue/metabolism ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology* ; Antioxidants/pharmacology* ; Connective Tissue/drug effects* ; Connective Tissue/metabolism ; Cytokines/genetics ; Cytokines/metabolism ; Enzyme-Linked Immunosorbent Assay ; Graves Ophthalmopathy/metabolism* ; Graves Ophthalmopathy/pathology ; Humans ; L-Lactate Dehydrogenase/metabolism ; Orbital Diseases/metabolism* ; Orbital Diseases/pathology ; Organ Culture Techniques ; Quercetin/pharmacology* ; RNA, Messenger/metabolism ; Real-Time Polymerase Chain Reaction
Abstract
PURPOSE: The authors previously reported that quercetin significantly inhibits interleukin (IL)-1β-induced increases of proinflammatory cytokines in cultured primary orbital fibroblasts from Graves' orbitopathy (GO). This study investigated the inhibitory effect of quercetin on inflammation in cultured whole orbital tissue.

METHODS: Orbital fat tissues from GO and normal samples were cultured with or without non-toxic concentrations of quercetin. Lactate dehydrogenase (LDH) release was used to identify non-toxic concentrations of quercetin. IL-6, IL-8, IL-1α, IL-1β and tumour necrosis factor alpha (TNFα) proteins were measured in tissue culture supernatants by ELISA, and gene transcript levels were determined using quantitative PCR, expressed as relative fold changes of threshold cycle value relative to the control group.

RESULTS: The maximal non-cytotoxic treatment of quercetin was 100 μM for 72 h, based on the considerably low LDH release with these conditions. IL-1β, IL-6 and TNFα protein levels corrected for tissue weight were significantly higher in supernatants of GO samples than normal controls (p<0.05). Quercetin reduced IL-6, IL-8 and TNFα protein production in supernatants of all GO samples (n=4) in a dose-dependent manner; however, only the reduction in IL-6 was statistically significant (p<0.05). Quercetin had a significant suppression of tissue IL-6, IL-8, IL-1β and TNFα mRNA expression in cultured orbital tissues from three GO samples relative to untreated control tissue (p<0.05).

CONCLUSIONS: Inhibition of proinflammatory cytokines by the natural product quercetin in both primary orbital fibroblasts and tissue culture provides the basis for its potential use as an anti-inflammatory agent in the treatment of GO.
Full Text
http://bjo.bmj.com/content/96/8/1117.long
DOI
22661653
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Jin Sook(윤진숙) ORCID logo https://orcid.org/0000-0002-8751-9467
Lee, Sang Yeul(이상열)
Lee, Eun Jig(이은직) ORCID logo https://orcid.org/0000-0002-9876-8370
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89829
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