Migration of Human Dermal Fibroblast is Affected by the Diameter of the Electrospun PLGA Fiber

Abstract

Cell migration is an essential activity of the cells in various biological phenomena such as embryonic development, wound healing of damaged tissue, capillary vascularization in angiogenesis and migration of leukocytes to kill the bacteria around the wound site. The properties of nanofibrous surface enhancing cell adhesion, proliferation, migration and differentiation are necessary for application in tissue engineering. Recently, fabricated scaffolds at the nanometer scale are very similar to the architecture of natural human tissue, because of the development of nanofibers. In this study, we observed different cell migration behaviors on PLGA nanofibers with different diameters. 0.4 μm and 1.4 μm PLGA fibers were fabricated by electrospinning. Adhesion of neonatal human dermal fibroblasts (nHDFs) on the PLGA scaffolds was quantified by MTT assay. Real time observation system was used to analyze the migration of nHDF on the 0.4 μm and 1.4 μm PLGA scaffolds. There are no significant differences in cell attachment between 0.4 μm and 1.4 μm PLGA nanofibers. However, the migration was affected by the thickness of the PLGA fiber. The cells were migrated along with the 0.4 μm PLGA fiber but did not cross 1.4 μm PLGA fiber. In this research, it would be evaluated that different diameter of electrospun PLGA fiber effect on the cell migration and proliferation, and it could be applied for the development of the fibrous scaffold in tissue engineering.