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Polydopamine-mediated immobilization of multiple bioactive molecules for the development of functional vascular graft materials

Authors
 Yu Bin Lee  ;  Young Min Shin  ;  Ji-hye Lee  ;  Indong Jun  ;  Jae Kyeong Kang  ;  Jong-Chul Park  ;  Heungsoo Shin 
Citation
 BIOMATERIALS, Vol.33(33) : 8343-8352, 2012 
Journal Title
BIOMATERIALS
ISSN
 0142-9612 
Issue Date
2012
MeSH
Human Umbilical Vein Endothelial Cells/cytology ; Humans ; Indoles/chemistry* ; Polyesters/chemistry ; Polymers/chemistry* ; Vascular Grafting/methods*
Keywords
Human umbilical vein endothelial cell ; Polydopamine coating ; Surface modification ; Endothelialization ; Dual factor immobilization
Abstract
In this study, we introduced a simple method for polydopamine-mediated immobilization of dual bioactive factors for the preparation of functionalized vascular graft materials. Polydopamine was deposited on elastic and biodegradable poly(lactic acid-co-ɛ-caprolactone) (PLCL) films, and a cell adhesive RGD-containing peptide and basic fibroblast growth factor were subsequently immobilized by simple dipping. We used an enzyme-linked immunosorbent assay and fluorescamine assay to confirm that we had stably immobilized bioactive molecules on the polydopamine-coated PLCL film in a reaction time-dependent manner. When human umbilical vein endothelial cells (HUVEC) were cultured on the prepared substrates, the number of adherent cells and proliferation of HUVEC for up to 14 days were greatest on the film immobilized with dual factors. On the other hand, the film immobilized with RGD peptide exhibited the highest migration speed compared to the other groups. The expression of cluster of differentiation 31 and von Willebrand factor, which indicates maturation of endothelial cells, was highly stimulated in the dual factor-immobilized group, and passively adsorbed factors showed a negligible effect. The immobilization of bioactive molecules inspired by polydopamine was successful, and adhesion, migration, proliferation and differentiation of HUVEC were synergistically accelerated by the presence of multiple signaling factors. Collectively, our results have demonstrated that a simple coating with polydopamine enables the immobilization of multiple bioactive molecules for preparation of polymeric functionalized vascular graft materials.
Full Text
http://www.sciencedirect.com/science/article/pii/S0142961212008873
DOI
10.1016/j.biomaterials.2012.08.011
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Kang, Jae Kyeong(강재경)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89744
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