7 288

Cited 5 times in

Increase of L-type Ca2+ current by protease-activated receptor 2 activation contributes to augmentation of spontaneous uterine contractility in pregnant rats

 Young-Hwan Kim  ;  Seungsoo Chung  ;  Young-Ho Lee  ;  Eok-Cheon Kim  ;  Duck-Sun Ahn 
 Biochemical and Biophysical Research Communications, Vol.418(1) : 167-172, 2012 
Journal Title
 Biochemical and Biophysical Research Communications 
Issue Date
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester/pharmacology ; Animals ; Calcium Channel Agonists/pharmacology ; Calcium Channels, L-Type/metabolism* ; Enzyme Activation ; Female ; Myometrium/drug effects ; Myometrium/metabolism ; Myometrium/physiology ; Pregnancy ; Protein Kinase C/metabolism ; Rats ; Rats, Sprague-Dawley ; Receptor, PAR-2/agonists ; Receptor, PAR-2/metabolism* ; Type C Phospholipases/metabolism ; Uterine Contraction* ; Uterus/drug effects ; Uterus/metabolism ; Uterus/physiology*
Preterm labor ; Uterine contractility ; Protease-activated receptor-2 ; L-type voltage-gated Ca2+ channel
We evaluated the effects of protease-activated receptor (PAR)-2 on spontaneous myometrial contraction (SMC) in isolated term pregnant myometrial strips of rat, and elucidated the cellular mechanisms of this effect using a conventional voltage-clamp method. In isometric tension measurements, trypsin and SL-NH(2), PAR-2 agonists, significantly augmented SMC in frequency and amplitude; however, boiled trypsin (BT) and LR-NH(2) had no effect on SMC. These stimulatory effects of PAR-2 agonists on SMC were nearly completely occluded by pre-application of Bay K 8644, an L-type voltage-gated Ca(2+) channel activator, thus showing the involvement of L-type voltage-gated Ca(2+) channels in PAR-2-induced augmentation of SMC. In addition, PAR-2 agonists significantly enhanced L-type voltage-gated Ca(2+) currents (I(Ca-L)), as measured by a conventional voltage-clamp method, and this increase was primarily mediated by activation of phospholipase C (PLC) and protein kinase C (PKC) via G-protein activation. Taken together, we have demonstrated that PAR-2 may actively regulate SMC during pregnancy by modulating Ca(2+) influx through L-type voltage-gated Ca(2+) channels, and that this increase of I(Ca-L) may be primarily mediated by PLC and PKC activation. These results suggest a cellular mechanism for the pathophysiological effects of PAR-2 activation on myometrial contractility during pregnancy and provide basic and theoretical information about developing new agents for the treatment of premature labor and other obstetric complications.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eok Cheon(김억천)
Kim, Young Hwan(김영환)
Ahn, Duk Sun(안덕선) ORCID logo https://orcid.org/0000-0001-9351-6951
Lee, Young Ho(이영호) ORCID logo https://orcid.org/0000-0002-5749-1045
Chung, Seung Soo(정승수) ORCID logo https://orcid.org/0000-0002-3119-9628
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.