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Increase of L-type Ca2+ current by protease-activated receptor 2 activation contributes to augmentation of spontaneous uterine contractility in pregnant rats
DC Field | Value | Language |
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dc.contributor.author | 김억천 | - |
dc.contributor.author | 김영환 | - |
dc.contributor.author | 안덕선 | - |
dc.contributor.author | 이영호 | - |
dc.contributor.author | 정승수 | - |
dc.date.accessioned | 2014-12-19T16:31:26Z | - |
dc.date.available | 2014-12-19T16:31:26Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/89691 | - |
dc.description.abstract | We evaluated the effects of protease-activated receptor (PAR)-2 on spontaneous myometrial contraction (SMC) in isolated term pregnant myometrial strips of rat, and elucidated the cellular mechanisms of this effect using a conventional voltage-clamp method. In isometric tension measurements, trypsin and SL-NH(2), PAR-2 agonists, significantly augmented SMC in frequency and amplitude; however, boiled trypsin (BT) and LR-NH(2) had no effect on SMC. These stimulatory effects of PAR-2 agonists on SMC were nearly completely occluded by pre-application of Bay K 8644, an L-type voltage-gated Ca(2+) channel activator, thus showing the involvement of L-type voltage-gated Ca(2+) channels in PAR-2-induced augmentation of SMC. In addition, PAR-2 agonists significantly enhanced L-type voltage-gated Ca(2+) currents (I(Ca-L)), as measured by a conventional voltage-clamp method, and this increase was primarily mediated by activation of phospholipase C (PLC) and protein kinase C (PKC) via G-protein activation. Taken together, we have demonstrated that PAR-2 may actively regulate SMC during pregnancy by modulating Ca(2+) influx through L-type voltage-gated Ca(2+) channels, and that this increase of I(Ca-L) may be primarily mediated by PLC and PKC activation. These results suggest a cellular mechanism for the pathophysiological effects of PAR-2 activation on myometrial contractility during pregnancy and provide basic and theoretical information about developing new agents for the treatment of premature labor and other obstetric complications. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester/pharmacology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Calcium Channel Agonists/pharmacology | - |
dc.subject.MESH | Calcium Channels, L-Type/metabolism* | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Myometrium/drug effects | - |
dc.subject.MESH | Myometrium/metabolism | - |
dc.subject.MESH | Myometrium/physiology | - |
dc.subject.MESH | Pregnancy | - |
dc.subject.MESH | Protein Kinase C/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Receptor, PAR-2/agonists | - |
dc.subject.MESH | Receptor, PAR-2/metabolism* | - |
dc.subject.MESH | Type C Phospholipases/metabolism | - |
dc.subject.MESH | Uterine Contraction* | - |
dc.subject.MESH | Uterus/drug effects | - |
dc.subject.MESH | Uterus/metabolism | - |
dc.subject.MESH | Uterus/physiology* | - |
dc.title | Increase of L-type Ca2+ current by protease-activated receptor 2 activation contributes to augmentation of spontaneous uterine contractility in pregnant rats | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Physiology (생리학) | - |
dc.contributor.googleauthor | Young-Hwan Kim | - |
dc.contributor.googleauthor | Seungsoo Chung | - |
dc.contributor.googleauthor | Young-Ho Lee | - |
dc.contributor.googleauthor | Eok-Cheon Kim | - |
dc.contributor.googleauthor | Duck-Sun Ahn | - |
dc.identifier.doi | 22244874 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00687 | - |
dc.contributor.localId | A00732 | - |
dc.contributor.localId | A02223 | - |
dc.contributor.localId | A02968 | - |
dc.contributor.localId | A03643 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 22244874 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X12000071 | - |
dc.subject.keyword | Preterm labor | - |
dc.subject.keyword | Uterine contractility | - |
dc.subject.keyword | Protease-activated receptor-2 | - |
dc.subject.keyword | L-type voltage-gated Ca2+ channel | - |
dc.contributor.alternativeName | Kim, Eok Cheon | - |
dc.contributor.alternativeName | Kim, Young Hwan | - |
dc.contributor.alternativeName | Ahn, Duk Sun | - |
dc.contributor.alternativeName | Lee, Young Ho | - |
dc.contributor.alternativeName | Chung, Seung Soo | - |
dc.contributor.affiliatedAuthor | Kim, Eok Cheon | - |
dc.contributor.affiliatedAuthor | Kim, Young Hwan | - |
dc.contributor.affiliatedAuthor | Ahn, Duk Sun | - |
dc.contributor.affiliatedAuthor | Lee, Young Ho | - |
dc.contributor.affiliatedAuthor | Chung, Seung Soo | - |
dc.citation.volume | 418 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 167 | - |
dc.citation.endPage | 172 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.418(1) : 167-172, 2012 | - |
dc.identifier.rimsid | 31854 | - |
dc.type.rims | ART | - |
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