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Increase of L-type Ca2+ current by protease-activated receptor 2 activation contributes to augmentation of spontaneous uterine contractility in pregnant rats

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dc.contributor.author김억천-
dc.contributor.author김영환-
dc.contributor.author안덕선-
dc.contributor.author이영호-
dc.contributor.author정승수-
dc.date.accessioned2014-12-19T16:31:26Z-
dc.date.available2014-12-19T16:31:26Z-
dc.date.issued2012-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/89691-
dc.description.abstractWe evaluated the effects of protease-activated receptor (PAR)-2 on spontaneous myometrial contraction (SMC) in isolated term pregnant myometrial strips of rat, and elucidated the cellular mechanisms of this effect using a conventional voltage-clamp method. In isometric tension measurements, trypsin and SL-NH(2), PAR-2 agonists, significantly augmented SMC in frequency and amplitude; however, boiled trypsin (BT) and LR-NH(2) had no effect on SMC. These stimulatory effects of PAR-2 agonists on SMC were nearly completely occluded by pre-application of Bay K 8644, an L-type voltage-gated Ca(2+) channel activator, thus showing the involvement of L-type voltage-gated Ca(2+) channels in PAR-2-induced augmentation of SMC. In addition, PAR-2 agonists significantly enhanced L-type voltage-gated Ca(2+) currents (I(Ca-L)), as measured by a conventional voltage-clamp method, and this increase was primarily mediated by activation of phospholipase C (PLC) and protein kinase C (PKC) via G-protein activation. Taken together, we have demonstrated that PAR-2 may actively regulate SMC during pregnancy by modulating Ca(2+) influx through L-type voltage-gated Ca(2+) channels, and that this increase of I(Ca-L) may be primarily mediated by PLC and PKC activation. These results suggest a cellular mechanism for the pathophysiological effects of PAR-2 activation on myometrial contractility during pregnancy and provide basic and theoretical information about developing new agents for the treatment of premature labor and other obstetric complications.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESH3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester/pharmacology-
dc.subject.MESHAnimals-
dc.subject.MESHCalcium Channel Agonists/pharmacology-
dc.subject.MESHCalcium Channels, L-Type/metabolism*-
dc.subject.MESHEnzyme Activation-
dc.subject.MESHFemale-
dc.subject.MESHMyometrium/drug effects-
dc.subject.MESHMyometrium/metabolism-
dc.subject.MESHMyometrium/physiology-
dc.subject.MESHPregnancy-
dc.subject.MESHProtein Kinase C/metabolism-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHReceptor, PAR-2/agonists-
dc.subject.MESHReceptor, PAR-2/metabolism*-
dc.subject.MESHType C Phospholipases/metabolism-
dc.subject.MESHUterine Contraction*-
dc.subject.MESHUterus/drug effects-
dc.subject.MESHUterus/metabolism-
dc.subject.MESHUterus/physiology*-
dc.titleIncrease of L-type Ca2+ current by protease-activated receptor 2 activation contributes to augmentation of spontaneous uterine contractility in pregnant rats-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Physiology (생리학)-
dc.contributor.googleauthorYoung-Hwan Kim-
dc.contributor.googleauthorSeungsoo Chung-
dc.contributor.googleauthorYoung-Ho Lee-
dc.contributor.googleauthorEok-Cheon Kim-
dc.contributor.googleauthorDuck-Sun Ahn-
dc.identifier.doi22244874-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00687-
dc.contributor.localIdA00732-
dc.contributor.localIdA02223-
dc.contributor.localIdA02968-
dc.contributor.localIdA03643-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid22244874-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X12000071-
dc.subject.keywordPreterm labor-
dc.subject.keywordUterine contractility-
dc.subject.keywordProtease-activated receptor-2-
dc.subject.keywordL-type voltage-gated Ca2+ channel-
dc.contributor.alternativeNameKim, Eok Cheon-
dc.contributor.alternativeNameKim, Young Hwan-
dc.contributor.alternativeNameAhn, Duk Sun-
dc.contributor.alternativeNameLee, Young Ho-
dc.contributor.alternativeNameChung, Seung Soo-
dc.contributor.affiliatedAuthorKim, Eok Cheon-
dc.contributor.affiliatedAuthorKim, Young Hwan-
dc.contributor.affiliatedAuthorAhn, Duk Sun-
dc.contributor.affiliatedAuthorLee, Young Ho-
dc.contributor.affiliatedAuthorChung, Seung Soo-
dc.citation.volume418-
dc.citation.number1-
dc.citation.startPage167-
dc.citation.endPage172-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.418(1) : 167-172, 2012-
dc.identifier.rimsid31854-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers

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