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Genetic and biochemical characterization of an acquired subgroup B3 metallo-β-lactamase gene, bla AIM-1, and its unique genetic context in Pseudomonas aeruginosa from Australia

Authors
 Dongeun Yong  ;  Mark A. Toleman  ;  Jan Bell  ;  Brett Ritchie  ;  Rachael Pratt  ;  Henry Ryleyd  ;  Timothy R. Walsh 
Citation
 ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Vol.56(12) : 6154-6159, 2012 
Journal Title
 ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 
ISSN
 0066-4804 
Issue Date
2012
MeSH
Adolescent ; Amino Acid Sequence ; Australia ; Bacterial Proteins/genetics* ; Bacterial Proteins/metabolism* ; DNA, Bacterial/genetics ; Diabetes Mellitus, Type 2/complications ; Electrophoresis, Gel, Pulsed-Field ; Endocarditis, Bacterial/complications ; Endocarditis, Bacterial/microbiology ; Female ; Genes, Bacterial ; Humans ; Hybridization, Genetic ; Kidney Failure, Chronic/complications ; Kinetics ; Leukemia, Myeloid/complications ; Male ; Methicillin-Resistant Staphylococcus aureus ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Pseudomonas Infections/complications ; Pseudomonas Infections/microbiology* ; Pseudomonas aeruginosa/drug effects* ; Staphylococcal Infections/complications ; Staphylococcal Infections/microbiology ; Streptococcus bovis ; beta-Lactamases/genetics* ; beta-Lactamases/metabolism*
Keywords
Adolescent ; Amino Acid Sequence ; Australia ; Bacterial Proteins/genetics* ; Bacterial Proteins/metabolism* ; DNA, Bacterial/genetics ; Diabetes Mellitus, Type 2/complications ; Electrophoresis, Gel, Pulsed-Field ; Endocarditis, Bacterial/complications ; Endocarditis, Bacterial/microbiology ; Female ; Genes, Bacterial ; Humans ; Hybridization, Genetic ; Kidney Failure, Chronic/complications ; Kinetics ; Leukemia, Myeloid/complications ; Male ; Methicillin-Resistant Staphylococcus aureus ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Pseudomonas Infections/complications ; Pseudomonas Infections/microbiology* ; Pseudomonas aeruginosa/drug effects* ; Staphylococcal Infections/complications ; Staphylococcal Infections/microbiology ; Streptococcus bovis ; beta-Lactamases/genetics* ; beta-Lactamases/metabolism*
Abstract
Three clinical Pseudomonas aeruginosa isolates (WCH2677, WCH2813, and WCH2837) isolated from the Women's and Children's Hospital, Adelaide, Australia, produced a metallo-β-lactamase (MBL)-positive Etest result. All isolates were PCR negative for known MBL genes. A gene bank was created, and an MBL gene, designated blaAIM-1, was cloned and fully characterized. The encoded enzyme, AIM-1, is a group B3 MBL that has the highest level of identity to THIN-B and L1. It is chromosomal and flanked by two copies (one intact and one truncated) of an ISCR element, ISCR15. Southern hybridization studies indicated the movement of both ISCR15 and blaAIM-1 within the three different clinical isolates. AIM-1 hydrolyzes most β-lactams, with the exception of aztreonam and, to a lesser extent, ceftazidime; however, it possesses significantly higher kcat values for cefepime and carbapenems than most other MBLs. AIM-1 was the first mobile group B3 enzyme detected and signals further problems for already beleaguered antimicrobial regimes to treat serious P. aeruginosa and other Gram-negative infections.
Files in This Item:
T201205578.pdf Download
DOI
22985886
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89577
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