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Genetic and biochemical characterization of an acquired subgroup B3 metallo-β-lactamase gene, bla AIM-1, and its unique genetic context in Pseudomonas aeruginosa from Australia

DC FieldValueLanguage
dc.contributor.author용동은-
dc.date.accessioned2014-12-19T16:27:54Z-
dc.date.available2014-12-19T16:27:54Z-
dc.date.issued2012-
dc.identifier.issn0066-4804-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/89577-
dc.description.abstractThree clinical Pseudomonas aeruginosa isolates (WCH2677, WCH2813, and WCH2837) isolated from the Women's and Children's Hospital, Adelaide, Australia, produced a metallo-β-lactamase (MBL)-positive Etest result. All isolates were PCR negative for known MBL genes. A gene bank was created, and an MBL gene, designated blaAIM-1, was cloned and fully characterized. The encoded enzyme, AIM-1, is a group B3 MBL that has the highest level of identity to THIN-B and L1. It is chromosomal and flanked by two copies (one intact and one truncated) of an ISCR element, ISCR15. Southern hybridization studies indicated the movement of both ISCR15 and blaAIM-1 within the three different clinical isolates. AIM-1 hydrolyzes most β-lactams, with the exception of aztreonam and, to a lesser extent, ceftazidime; however, it possesses significantly higher kcat values for cefepime and carbapenems than most other MBLs. AIM-1 was the first mobile group B3 enzyme detected and signals further problems for already beleaguered antimicrobial regimes to treat serious P. aeruginosa and other Gram-negative infections.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfANTIMICROBIAL AGENTS AND CHEMOTHERAPY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdolescent-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAustralia-
dc.subject.MESHBacterial Proteins/genetics*-
dc.subject.MESHBacterial Proteins/metabolism*-
dc.subject.MESHDNA, Bacterial/genetics-
dc.subject.MESHDiabetes Mellitus, Type 2/complications-
dc.subject.MESHElectrophoresis, Gel, Pulsed-Field-
dc.subject.MESHEndocarditis, Bacterial/complications-
dc.subject.MESHEndocarditis, Bacterial/microbiology-
dc.subject.MESHFemale-
dc.subject.MESHGenes, Bacterial-
dc.subject.MESHHumans-
dc.subject.MESHHybridization, Genetic-
dc.subject.MESHKidney Failure, Chronic/complications-
dc.subject.MESHKinetics-
dc.subject.MESHLeukemia, Myeloid/complications-
dc.subject.MESHMale-
dc.subject.MESHMethicillin-Resistant Staphylococcus aureus-
dc.subject.MESHMicrobial Sensitivity Tests-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPseudomonas Infections/complications-
dc.subject.MESHPseudomonas Infections/microbiology*-
dc.subject.MESHPseudomonas aeruginosa/drug effects*-
dc.subject.MESHStaphylococcal Infections/complications-
dc.subject.MESHStaphylococcal Infections/microbiology-
dc.subject.MESHStreptococcus bovis-
dc.subject.MESHbeta-Lactamases/genetics*-
dc.subject.MESHbeta-Lactamases/metabolism*-
dc.titleGenetic and biochemical characterization of an acquired subgroup B3 metallo-β-lactamase gene, bla AIM-1, and its unique genetic context in Pseudomonas aeruginosa from Australia-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorDongeun Yong-
dc.contributor.googleauthorMark A. Toleman-
dc.contributor.googleauthorJan Bell-
dc.contributor.googleauthorBrett Ritchie-
dc.contributor.googleauthorRachael Pratt-
dc.contributor.googleauthorHenry Ryleyd-
dc.contributor.googleauthorTimothy R. Walsh-
dc.identifier.doi22985886-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02423-
dc.relation.journalcodeJ00189-
dc.identifier.eissn1098-6596-
dc.identifier.pmid22985886-
dc.subject.keywordAdolescent-
dc.subject.keywordAmino Acid Sequence-
dc.subject.keywordAustralia-
dc.subject.keywordBacterial Proteins/genetics*-
dc.subject.keywordBacterial Proteins/metabolism*-
dc.subject.keywordDNA, Bacterial/genetics-
dc.subject.keywordDiabetes Mellitus, Type 2/complications-
dc.subject.keywordElectrophoresis, Gel, Pulsed-Field-
dc.subject.keywordEndocarditis, Bacterial/complications-
dc.subject.keywordEndocarditis, Bacterial/microbiology-
dc.subject.keywordFemale-
dc.subject.keywordGenes, Bacterial-
dc.subject.keywordHumans-
dc.subject.keywordHybridization, Genetic-
dc.subject.keywordKidney Failure, Chronic/complications-
dc.subject.keywordKinetics-
dc.subject.keywordLeukemia, Myeloid/complications-
dc.subject.keywordMale-
dc.subject.keywordMethicillin-Resistant Staphylococcus aureus-
dc.subject.keywordMicrobial Sensitivity Tests-
dc.subject.keywordMolecular Sequence Data-
dc.subject.keywordPseudomonas Infections/complications-
dc.subject.keywordPseudomonas Infections/microbiology*-
dc.subject.keywordPseudomonas aeruginosa/drug effects*-
dc.subject.keywordStaphylococcal Infections/complications-
dc.subject.keywordStaphylococcal Infections/microbiology-
dc.subject.keywordStreptococcus bovis-
dc.subject.keywordbeta-Lactamases/genetics*-
dc.subject.keywordbeta-Lactamases/metabolism*-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.citation.volume56-
dc.citation.number12-
dc.citation.startPage6154-
dc.citation.endPage6159-
dc.identifier.bibliographicCitationANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Vol.56(12) : 6154-6159, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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