6 464

Cited 23 times in

Regulation of haemolysin(VvhA) production by ferric uptake regulator (Fur) in Vibrio vulnificus: repression of vvhA transcription by Fur and proteolysis of VVhA by Fur-repressive exoproteases

Authors
 Hyun-Jung Lee  ;  Jeong-A Kim  ;  Mi-Ae Lee  ;  Soon-Jung Park  ;  Kyu-Ho Lee 
Citation
 MOLECULAR MICROBIOLOGY, Vol.88(4) : 813-826, 2013 
Journal Title
MOLECULAR MICROBIOLOGY
ISSN
 0950-382X 
Issue Date
2013
MeSH
Bacterial Proteins/metabolism* ; Exopeptidases/metabolism* ; Gene Expression Regulation, Bacterial* ; Operator Regions, Genetic ; Protein Binding ; Proteolysis* ; Repressor Proteins/metabolism* ; Transcription, Genetic* ; Vibrio vulnificus/genetics* ; Vibrio vulnificus/metabolism
Keywords
Bacterial Proteins/metabolism* ; Exopeptidases/metabolism* ; Gene Expression Regulation, Bacterial* ; Operator Regions, Genetic ; Protein Binding ; Proteolysis* ; Repressor Proteins/metabolism* ; Transcription, Genetic* ; Vibrio vulnificus/genetics* ; Vibrio vulnificus/metabolism
Abstract
VvhA produced by Vibrio vulnificus exhibits cytolytic activity to human cells including erythrocytes. Since haemolysis by VvhA may provide iron for bacterial growth and pathogenicity, we investigated the expression of VvhA to elucidate the regulatory roles of Fur, a major transcription factor controlling iron-homeostasis. Fur repressed the transcription of vvhBA operon via binding to the promoter region. However, haemolysin content and haemolytic activity were lowered in cell-free supernatant of fur mutant. This discrepancy between the levels of vvhA transcript and VvhA protein in fur mutant was caused by exoproteolytic activities of the elastase VvpE and another metalloprotease VvpM, which were also regulated by Fur. vvpE gene expression was repressed by Fur via binding to the Fur-box homologous region. Regulation of VvpM expression by Fur did not occur at the level of vvpM transcription. In vitro proteolysis assays showed that both proteases efficiently degraded VvhA. In addition, the extracellular levels of VvhA were higher in culture supernatants of vvpE or vvpM mutants than in the wild type. Thus this study demonstrates that Fur regulates haemolysin production at the transcription level of the vvhBA operon and at the post-translation level by regulating the expressions of two VvhA-degrading exoproteases, VvpE and VvpM.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/mmi.12224/abstract
DOI
10.1111/mmi.12224
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89307
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links