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Proliferation and Functional Assessment of Pseudo-islets With the Use of Pancreatic Endocrine Cells

Authors
 M.K. Ju  ;  J.H. Jeong  ;  J.I. Lee  ;  Y.S. Kim  ;  M.S. Kim 
Citation
 TRANSPLANTATION PROCEEDINGS, Vol.45(5) : 1885-1888, 2013 
Journal Title
 TRANSPLANTATION PROCEEDINGS 
ISSN
 0041-1345 
Issue Date
2013
MeSH
Animals ; Base Sequence ; Cell Differentiation* ; Cell Proliferation* ; DNA Primers ; Glucose Tolerance Test ; Islets of Langerhans/cytology* ; Male ; Pancreas/cytology* ; Rats ; Rats, Inbred Lew ; Reverse Transcriptase Polymerase Chain Reaction
Keywords
Animals ; Base Sequence ; Cell Differentiation* ; Cell Proliferation* ; DNA Primers ; Glucose Tolerance Test ; Islets of Langerhans/cytology* ; Male ; Pancreas/cytology* ; Rats ; Rats, Inbred Lew ; Reverse Transcriptase Polymerase Chain Reaction
Abstract
Many obstacles beset islet transplantation, particularly insufficient tissue mass. Previously, we reported production of pseudo-islets. In addition, there have been reports in which coculture with pancreatic islet and bone marrow mesenchymal stem cells (BMSCs) demonstrated positive effects on pancreatic islet function. The purpose of this study was to perform morphologic and functional evaluations of pancreatic pseudo-islets cocultured with BMSCs. Pancreatic endocrine cells (PECs) were collected with a previously reported method; bone marrow was aspirated from the rat femur. Subsequently, PECs and BMSCs cocultured at high density on low-cell-binding culture dishes kept suspended by shaking. The functionality and characteristics of the mixed cell complexes were evaluated by glucose challenge, insulin enzyme-linked immunosorbent assay, reverse-transcription polymerase chain reaction, and immunohistochemistry. Through expansion for 2 weeks in continuous culture passages, ∼1 million PECs were recovered after aggregation. They presented spherical shapes and sizes similar to naïve islets, according to phase-contrast microscopy. The spheroid aggregates of pancreatic islet cells and BMSCs showed fortified functions and maintained viability. In conclusion, PECs served as a cell source for pseudo-islets, which were both morphologically and genetically similar to naïve islets. We also suggest a manufacturing method for mixed cellular complexes from 2 different origins that can improve secretion ability and cell differentiation.
Full Text
http://www.sciencedirect.com/science/article/pii/S0041134513000869
DOI
10.1016/j.transproceed.2012.12.029
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Soo(김명수) ORCID logo https://orcid.org/0000-0002-8975-8381
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Joo, Man Ki(주만기) ORCID logo https://orcid.org/0000-0002-4112-7003
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/89201
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