3 176

Cited 3 times in

Proliferation and Functional Assessment of Pseudo-islets With the Use of Pancreatic Endocrine Cells

DC FieldValueLanguage
dc.contributor.author김명수-
dc.contributor.author김유선-
dc.contributor.author주만기-
dc.date.accessioned2014-12-18T09:59:09Z-
dc.date.available2014-12-18T09:59:09Z-
dc.date.issued2013-
dc.identifier.issn0041-1345-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/89201-
dc.description.abstractMany obstacles beset islet transplantation, particularly insufficient tissue mass. Previously, we reported production of pseudo-islets. In addition, there have been reports in which coculture with pancreatic islet and bone marrow mesenchymal stem cells (BMSCs) demonstrated positive effects on pancreatic islet function. The purpose of this study was to perform morphologic and functional evaluations of pancreatic pseudo-islets cocultured with BMSCs. Pancreatic endocrine cells (PECs) were collected with a previously reported method; bone marrow was aspirated from the rat femur. Subsequently, PECs and BMSCs cocultured at high density on low-cell-binding culture dishes kept suspended by shaking. The functionality and characteristics of the mixed cell complexes were evaluated by glucose challenge, insulin enzyme-linked immunosorbent assay, reverse-transcription polymerase chain reaction, and immunohistochemistry. Through expansion for 2 weeks in continuous culture passages, ∼1 million PECs were recovered after aggregation. They presented spherical shapes and sizes similar to naïve islets, according to phase-contrast microscopy. The spheroid aggregates of pancreatic islet cells and BMSCs showed fortified functions and maintained viability. In conclusion, PECs served as a cell source for pseudo-islets, which were both morphologically and genetically similar to naïve islets. We also suggest a manufacturing method for mixed cellular complexes from 2 different origins that can improve secretion ability and cell differentiation.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfTRANSPLANTATION PROCEEDINGS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBase Sequence-
dc.subject.MESHCell Differentiation*-
dc.subject.MESHCell Proliferation*-
dc.subject.MESHDNA Primers-
dc.subject.MESHGlucose Tolerance Test-
dc.subject.MESHIslets of Langerhans/cytology*-
dc.subject.MESHMale-
dc.subject.MESHPancreas/cytology*-
dc.subject.MESHRats-
dc.subject.MESHRats, Inbred Lew-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.titleProliferation and Functional Assessment of Pseudo-islets With the Use of Pancreatic Endocrine Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorM.K. Ju-
dc.contributor.googleauthorJ.H. Jeong-
dc.contributor.googleauthorJ.I. Lee-
dc.contributor.googleauthorY.S. Kim-
dc.contributor.googleauthorM.S. Kim-
dc.identifier.doi10.1016/j.transproceed.2012.12.029-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00785-
dc.contributor.localIdA03949-
dc.contributor.localIdA00424-
dc.relation.journalcodeJ02755-
dc.identifier.eissn1873-2623-
dc.identifier.pmid23769063-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0041134513000869-
dc.subject.keywordAnimals-
dc.subject.keywordBase Sequence-
dc.subject.keywordCell Differentiation*-
dc.subject.keywordCell Proliferation*-
dc.subject.keywordDNA Primers-
dc.subject.keywordGlucose Tolerance Test-
dc.subject.keywordIslets of Langerhans/cytology*-
dc.subject.keywordMale-
dc.subject.keywordPancreas/cytology*-
dc.subject.keywordRats-
dc.subject.keywordRats, Inbred Lew-
dc.subject.keywordReverse Transcriptase Polymerase Chain Reaction-
dc.contributor.alternativeNameKim, Myoung Soo-
dc.contributor.alternativeNameKim, Yu Seun-
dc.contributor.alternativeNameJoo, Man Ki-
dc.contributor.affiliatedAuthorKim, Yu Seun-
dc.contributor.affiliatedAuthorJoo, Man Ki-
dc.contributor.affiliatedAuthorKim, Myoung Soo-
dc.rights.accessRightsnot free-
dc.citation.volume45-
dc.citation.number5-
dc.citation.startPage1885-
dc.citation.endPage1888-
dc.identifier.bibliographicCitationTRANSPLANTATION PROCEEDINGS, Vol.45(5) : 1885-1888, 2013-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.