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The effects of enhancing the surface energy of a polystyrene plate by air atmospheric pressure plasma jet on early attachment of fibroblast under moving incubation

Authors
 Jung-Hwan Lee  ;  Jae-Sung Kwon  ;  Yong-Hee Kim  ;  Eun-Ha Choi  ;  Kwang-Mahn Kim  ;  Kyoung-Nam Kim 
Citation
 THIN SOLID FILMS, Vol.547 : 99-105, 2013 
Journal Title
 THIN SOLID FILMS 
ISSN
 0040-6090 
Issue Date
2013
Keywords
Air non-thermal atmospheric pressure plasma jet ; Surface energy ; Moving incubation ; Mouse fibroblast ; Polystyrene plate ; Hydrocarbon ; Vinculin
Abstract
Enhancing the biomaterial surface energy has received wide attention as a possible option for improving cell adhesion. Non-thermal atmospheric pressure plasma jets (APPJ) have been introduced to medical fields as one of the on-site treatment methods to enhance surface energy before implantation. The aim of this study is to evaluate the effect of enhancing the surface energy of a polystyrene plate by air APPJ on the early attachment of fibroblasts under moving incubation. In addition, the range of plasma jet effect was measured for easy on-site application. After 2 min of air APPJ treatment, mouse fibroblasts (L929) were separately seeded through the prepared holes on the treated polystyrene plate as well as as-received polystyrene plate. Cells were allowed to attach for 30 min or 4 h under 70 revolutions per minute. The surface characteristic results confirmed the increase of surface energy six times after air APPJ treatment with the Owens–Wendt method. Minimal change of surface temperature and amine functional group without roughness changes were detected. In the case of a short attachment time (30 min), the attached L929 were only found on air APPJ-treated center surface. In 4 h attachment time, cells were attached with vinculin focal adhesion and elongated actin filament on the both the right and left side surfaces from the center area which was treated by the plasma jet flame. In light of this study, air APPJ could be useful for early-rapid attachment of mouse fibroblasts on polystyrene plates under moving incubation. Future studies can examine its on-site use with biomaterials.
Full Text
http://www.sciencedirect.com/science/article/pii/S0040609013007414
DOI
10.1016/j.tsf.2013.04.105
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Jae-Sung(권재성) ORCID logo https://orcid.org/0000-0001-9803-7730
Kim, Kyoung Nam(김경남)
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Lee, Jung Hwan(이정환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88904
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