102 166

Cited 2 times in

Effect of cholesterol depletion on interleukin-8 production in human respiratory epithelial cells

Authors
 Min Jung Kim  ;  Jung Yeon Hong  ;  Kyung Eun Lee  ;  Kyung Won Kim  ;  Myung Hyun Sohn  ;  Kyu-Earn Kim 
Citation
 Allergy Asthma & Immunology Research, Vol.5(6) : 402-408, 2013 
Journal Title
 Allergy Asthma & Immunology Research 
ISSN
 2092-7355 
Issue Date
2013
Abstract
PURPOSE: The lipid entities of cell membranes are components of the immune system and important mediators of inflammation. Despite increasing interest in the function of epithelial cells in inflammation, the role of cholesterol in this process has not been described. Here, we investigated the effect of cholesterol depletion on the inflammatory process in airway epithelial cells via the expression of interleukin (IL)-8 as a marker of inflammation. METHODS: A 549 cells were treated with 0.5% methyl-β-cyclodextrin as a selective cholesterol extractor. The IL-8 level was assessed by enzyme-linked immunosorbent assay and reassessed after cholesterol repletion. Mitogen-activated protein kinase (MAPK) inhibitors were used to determine the upstream signaling pathway for IL-8 production in cholesterol-depleted cells. RESULTS: We found a relationship between the amount of cholesterol in A 549 cells and inflammation of the airway. IL-8 production was increased in cholesterol-depleted A 549 cells and restored by cholesterol repletion. IL-8 production was decreased by pretreatment with the extracellular signal-regulated kinase (ERK) inhibitor U0126 but not with JNK inhibitor II or the p38 MAPK inhibitor SB202190. CONCLUSIONS: Our findings suggest that inflammatory responses are increased in cholesterol-depleted epithelial cells via the MAPK signaling system, predominantly by the ERK pathway. We conclude that the lipid components of airwayepithelial cells may play a role in the inflammatory process
Files in This Item:
T201305207.pdf Download
DOI
10.4168/aair.2013.5.6.402
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실)
Yonsei Authors
김경원(Kim, Kyung Won) ORCID logo https://orcid.org/0000-0003-4529-6135
김규언(Kim, Kyu Earn)
김민정(Kim, Min Jung) ORCID logo https://orcid.org/0000-0002-5634-9709
손명현(Sohn, Myung Hyun) ORCID logo https://orcid.org/0000-0002-2478-487X
이경은(Lee, Kyung Eun)
홍정연(Hong, Jung Yeon) ORCID logo https://orcid.org/0000-0003-0406-9956
Export
RIS (EndNote)
XLS (Excel)
XML
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88833
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse