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Fast temporal detection of intracellular hydrogen peroxide by HyPer

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dc.contributor.author신동민-
dc.contributor.author양유미-
dc.contributor.author이성준-
dc.date.accessioned2014-12-18T09:40:21Z-
dc.date.available2014-12-18T09:40:21Z-
dc.date.issued2013-
dc.identifier.issn1226-7155-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/88610-
dc.description.abstractHyPer is the genetically encoded biosensor of intracellular hydrogen peroxide (H2O2), the most stable of the reactive oxygen species (ROS) generated by living cells. HyPer has a high sensitivity and specificity for detecting intracellular H2O2 by confocal laser microscopy. However, it was not known whether high speed ratiometric imaging of H2O2 by HyPer is possible. We thus investigated the sensitivity of HyPer in detecting changes to the intracellular H2O2 levels in HEK293 and PC12 cells using a microfluorometer imaging system. Increase in the HyPer ratio were clearly evident on stimulations of more than 100 μM H2O2 and fast changes in the HyPer ratio were observed on ratiometric fluorescent images after H2O2 treatment. These results suggest that HyPer is a potent biosensor of the fast temporal production of intracellular H2O2.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfInternational Journal of Oral Biology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleFast temporal detection of intracellular hydrogen peroxide by HyPer-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.department생체방어연구센터-
dc.contributor.googleauthorYu-Mi Yang-
dc.contributor.googleauthorSung Jun Lee-
dc.contributor.googleauthorDong Min Shin-
dc.identifier.doi10.11620/IJOB.2013.38.4.169-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02091-
dc.contributor.localIdA02870-
dc.contributor.localIdA05148-
dc.relation.journalcodeJ01144-
dc.identifier.pmidHyPer ; H2O2 ; ROS ; high speed detection-
dc.subject.keywordHyPer-
dc.subject.keywordH2O2-
dc.subject.keywordROS-
dc.subject.keywordhigh speed detection-
dc.contributor.alternativeNameShin, Dong Min-
dc.contributor.alternativeNameYang, Yu-Mi-
dc.contributor.alternativeNameLee, Sung Jun-
dc.contributor.affiliatedAuthorShin, Dong Min-
dc.contributor.affiliatedAuthorLee, Sung Jun-
dc.contributor.affiliatedAuthorYang, Yu Mi-
dc.rights.accessRightsfree-
dc.citation.volume38-
dc.citation.number4-
dc.citation.startPage169-
dc.citation.endPage173-
dc.identifier.bibliographicCitationInternational Journal of Oral Biology, Vol.38(4) : 169-173, 2013-
dc.identifier.rimsid33353-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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