Cited 0 times in
Fast temporal detection of intracellular hydrogen peroxide by HyPer
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신동민 | - |
dc.contributor.author | 양유미 | - |
dc.contributor.author | 이성준 | - |
dc.date.accessioned | 2014-12-18T09:40:21Z | - |
dc.date.available | 2014-12-18T09:40:21Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/88610 | - |
dc.description.abstract | HyPer is the genetically encoded biosensor of intracellular hydrogen peroxide (H2O2), the most stable of the reactive oxygen species (ROS) generated by living cells. HyPer has a high sensitivity and specificity for detecting intracellular H2O2 by confocal laser microscopy. However, it was not known whether high speed ratiometric imaging of H2O2 by HyPer is possible. We thus investigated the sensitivity of HyPer in detecting changes to the intracellular H2O2 levels in HEK293 and PC12 cells using a microfluorometer imaging system. Increase in the HyPer ratio were clearly evident on stimulations of more than 100 μM H2O2 and fast changes in the HyPer ratio were observed on ratiometric fluorescent images after H2O2 treatment. These results suggest that HyPer is a potent biosensor of the fast temporal production of intracellular H2O2. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | International Journal of Oral Biology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Fast temporal detection of intracellular hydrogen peroxide by HyPer | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | 생체방어연구센터 | - |
dc.contributor.googleauthor | Yu-Mi Yang | - |
dc.contributor.googleauthor | Sung Jun Lee | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.identifier.doi | 10.11620/IJOB.2013.38.4.169 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02870 | - |
dc.contributor.localId | A05148 | - |
dc.relation.journalcode | J01144 | - |
dc.identifier.pmid | HyPer ; H2O2 ; ROS ; high speed detection | - |
dc.subject.keyword | HyPer | - |
dc.subject.keyword | H2O2 | - |
dc.subject.keyword | ROS | - |
dc.subject.keyword | high speed detection | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Yang, Yu-Mi | - |
dc.contributor.alternativeName | Lee, Sung Jun | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Lee, Sung Jun | - |
dc.contributor.affiliatedAuthor | Yang, Yu Mi | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 38 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 169 | - |
dc.citation.endPage | 173 | - |
dc.identifier.bibliographicCitation | International Journal of Oral Biology, Vol.38(4) : 169-173, 2013 | - |
dc.identifier.rimsid | 33353 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.