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A comparison of inflammatory mediator expression between palmoplantar pustulosis and pompholyx

Authors
 D.Y. Kim  ;  J.Y. Kim  ;  T.G. Kim  ;  J.E. Kwon  ;  H. Sohn  ;  J. Park  ;  B.J. Lim  ;  S.H. Oh 
Citation
 JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY, Vol.27(12) : 1559-1565, 2013 
Journal Title
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
ISSN
 0926-9959 
Issue Date
2013
MeSH
Base Sequence ; DNA Primers ; Eczema, Dyshidrotic/metabolism* ; Gene Expression Profiling ; Humans ; Inflammation Mediators/metabolism* ; Prospective Studies ; Real-Time Polymerase Chain Reaction
Keywords
Base Sequence ; DNA Primers ; Eczema, Dyshidrotic/metabolism* ; Gene Expression Profiling ; Humans ; Inflammation Mediators/metabolism* ; Prospective Studies ; Real-Time Polymerase Chain Reaction
Abstract
BACKGROUND:
Both palmoplantar pustulosis (PPP) and pompholyx are clinically characterized by acute eruptions of vesicles or pustules on the palms or soles.
OBJECTIVES:
This study aims to compare the expression of certain inflammatory mediator genes and proteins between patients with PPP and pompholyx using skin tissue samples.
METHODS:
Skin biopsies obtained from lesional skin from patients with PPP (n = 7) and pompholyx (n = 5) were analysed by quantitative RT-PCR to measure the mRNA levels of nine genes, including IL-4, IL-8, IL-9, IL-17, IL-22, IFN-γ, CCL-20, granzyme and perforin. For immunohistochemical analysis, 34 paraffin-embedded skin specimens (PPP, n = 22; pompholyx, n = 12) were stained with anti-IL-8, IL-17A, IL-22 and granzyme B antibodies.
RESULTS:
Of genes analysed, IL-8 and IL-17A mRNA expression levels were significantly higher in the PPP group than the pompholyx group (P = 0.012 in both), whereas the mRNA expression of granzyme B was significantly higher in pompholyx when compared with PPP (P = 0.004). Regarding the IL-17A immunohistochemical staining, tissue from the PPP lesions contained significantly more IL-17A(+) cells in both the epidermis and papillary dermis when compared with pompholyx (P < 0.001 and P = 0.019 respectively). Moreover, the intensity of the IL-8 immunoreactivity was also greater in the PPP skin lesions than the pompholyx tissue (P < 0.001).
CONCLUSIONS:
IL-8 and IL-17A, both are increased in PPP tissue, may represent important immunologic mediators that help to differentiate this clinical entity from pompholyx. This study may provide useful clues in distinguishing PPP from pompholyx, as well as helping to understand the pathogeneses of these two diseases.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/jdv.12203/abstract
DOI
10.1111/jdv.12203
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Do Young(김도영)
Kim, Tae-Gyun(김태균) ORCID logo https://orcid.org/0000-0002-2116-4579
Sohn, Hyo Jung(손효정)
Oh, Sang Ho(오상호) ORCID logo https://orcid.org/0000-0002-4477-1400
Lim, Beom Jin(임범진) ORCID logo https://orcid.org/0000-0003-2856-0133
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/88514
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