Cited 19 times in
Thrombolytic Effects of the Snake Venom Disintegrin Saxatilin Determined by Novel Assessment Methods: A FeCl3-Induced Thrombosis Model in Mice.
DC Field | Value | Language |
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dc.contributor.author | 강성수 | - |
dc.contributor.author | 권일 | - |
dc.contributor.author | 김영대 | - |
dc.contributor.author | 남효석 | - |
dc.contributor.author | 양승희 | - |
dc.contributor.author | 허지회 | - |
dc.contributor.author | 홍성유 | - |
dc.date.accessioned | 2014-12-18T09:36:00Z | - |
dc.date.available | 2014-12-18T09:36:00Z | - |
dc.date.issued | 2013 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/88474 | - |
dc.description.abstract | Saxatilin, a novel disintegrin purified and cloned from the venom of the Korean snake Gloydius saxatilis, strongly inhibits activation and aggregation of platelets. Glycoprotein (GP) IIb/IIIa receptor antagonists can resolve thrombus, so saxatilin might also have thrombolytic effects. We investigated the thrombolytic effects of saxatilin in mice using a ferric chloride-induced carotid arterial thrombosis model. Thrombotic occlusion and thrombus resolution were evaluated quantitatively by measuring blood flow in the carotid artery with an ultrasonic flow meter and calculating the degree of flow restoration on a minute-by-minute basis; results were confirmed by histological examination. Saxatilin dissolved thrombi in a dose-dependent manner. Saxatilin at 5 mg/kg restored blood flow to baseline levels. As saxatilin dose increased, time to recanalization decreased. A bolus injection of 10% of a complete dose with continuous infusion of the remaining dose for 60 minutes resulted in effective recanalization without reocclusion. The thrombolytic effect of saxatilin was also demonstrated in vitro using platelet aggregometry by administering saxatilin in preformed thrombi. Bleeding complications were observed in 2 of 71 mice that received saxatilin. Fibrin/fibrinogen zymography and platelet aggregometry studies indicated that saxatilin does not have fibrinolytic activity, but exerted its action on platelets. Integrin-binding assays showed that saxatilin inhibited multiple integrins, specifically α2bβ3 (GP IIb/IIIa), α5β1, αvβ3, αvβ1, and αvβ5, which act on platelet adhesion/aggregation. Saxatilin inhibited multiple integrins by acting on platelets, and was safe and effective in resolving thrombi in mice. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Thrombolytic Effects of the Snake Venom Disintegrin Saxatilin Determined by Novel Assessment Methods: A FeCl3-Induced Thrombosis Model in Mice. | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Yonsei Cardiovascular Research Institute (심혈관연구소) | - |
dc.contributor.googleauthor | Il Kwon | - |
dc.contributor.googleauthor | Sung-Yu Hong | - |
dc.contributor.googleauthor | Young Dae Kim | - |
dc.contributor.googleauthor | Hyo Suk Nam | - |
dc.contributor.googleauthor | Sungsoo Kang | - |
dc.contributor.googleauthor | Seung-Hee Yang | - |
dc.contributor.googleauthor | Ji Hoe Heo | - |
dc.identifier.doi | 10.1371/journal.pone.0081165 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00039 | - |
dc.contributor.localId | A00245 | - |
dc.contributor.localId | A00702 | - |
dc.contributor.localId | A01273 | - |
dc.contributor.localId | A02296 | - |
dc.contributor.localId | A04369 | - |
dc.contributor.localId | A04400 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.contributor.alternativeName | Kang, Sung Soo | - |
dc.contributor.alternativeName | Kwon, Il | - |
dc.contributor.alternativeName | Kim, Young Dae | - |
dc.contributor.alternativeName | Nam, Hyo Suk | - |
dc.contributor.alternativeName | Yang, Seung Hee | - |
dc.contributor.alternativeName | Heo, Ji Hoe | - |
dc.contributor.alternativeName | Hong, Sung Yu | - |
dc.contributor.affiliatedAuthor | Kang, Sung Soo | - |
dc.contributor.affiliatedAuthor | Kwon, Il | - |
dc.contributor.affiliatedAuthor | Kim, Young Dae | - |
dc.contributor.affiliatedAuthor | Nam, Hyo Suk | - |
dc.contributor.affiliatedAuthor | Yang, Seung Hee | - |
dc.contributor.affiliatedAuthor | Heo, Ji Hoe | - |
dc.contributor.affiliatedAuthor | Hong, Sung Yu | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 8 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | e81165 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.8(11) : e81165, 2013 | - |
dc.identifier.rimsid | 34053 | - |
dc.type.rims | ART | - |
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