Cited 11 times in
Overexpressed proprotein convertase 1/3 induces an epithelial-mesenchymal transition in airway epithelium
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 손명현 | - |
dc.contributor.author | 윤주헌 | - |
dc.contributor.author | 이다형 | - |
dc.contributor.author | 이상남 | - |
dc.date.accessioned | 2014-12-18T09:31:36Z | - |
dc.date.available | 2014-12-18T09:31:36Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0903-1936 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/88333 | - |
dc.description.abstract | The proprotein convertases (PCs) are serine proteases responsible for the proteolytic maturation of many precursor proteins involved in upper airway remodelling during nasal polyposis. We have previously found that PC1/3 is expressed in human nasal mucosa. However, whether PC1/3 is related to nasal polyp formation has not been investigated. To gain insight into the functional role of PC1/3 in nasal polyps, we determined PC1/3 expression in nasal polyps by immunostaining, Western blotting and enzyme assays and generated stable cells expressing PC1/3 using airway epithelial cell line NCI-H292. Nasal polyps exhibit increased PC1/3 expression compared to normal nasal mucosa. PC1/3 was expressed in neuroendocrine cells in normal nasal mucosa and it was also expressed in goblet and ciliated cells in nasal polyps. NCI-H292 cells stably expressing PC1/3 displayed morphological changes, enhanced cell proliferation and migration, downregulation of E-cadherin and cytokeratins and upregulation of N-cadherin, vimentin, matrix metalloproteinase-2, collagen-I, snail and twist. Importantly, PC1/3 expression was positively correlated with epithelial-mesenchymal transition in cultured human nasal epithelial cells and in nasal polyps. Taken together, our data suggest that PC1/3 overexpression induces morphological and phenotypic epithelial-mesenchymal transition changes of airway epithelial cells and these changes may contribute to the pathogenesis of nasal polyps. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | EUROPEAN RESPIRATORY JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Epithelial Cells/cytology* | - |
dc.subject.MESH | Epithelial-Mesenchymal Transition* | - |
dc.subject.MESH | Epithelium/metabolism* | - |
dc.subject.MESH | Gene Expression Profiling | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Goblet Cells/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Inflammation | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Nasal Mucosa/metabolism | - |
dc.subject.MESH | Nasal Polyps/metabolism* | - |
dc.subject.MESH | Neuroendocrine Cells/cytology | - |
dc.subject.MESH | Phenotype | - |
dc.subject.MESH | Proprotein Convertase 1/physiology* | - |
dc.subject.MESH | Respiratory System/cytology | - |
dc.subject.MESH | Sinusitis/metabolism | - |
dc.subject.MESH | Wound Healing | - |
dc.title | Overexpressed proprotein convertase 1/3 induces an epithelial-mesenchymal transition in airway epithelium | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | 생체방어연구센터 | - |
dc.contributor.googleauthor | Sang-Nam Lee | - |
dc.contributor.googleauthor | Da-Hyung Lee | - |
dc.contributor.googleauthor | Myung Hyun Sohn | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.identifier.doi | 10.1183/09031936.00100412 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01967 | - |
dc.contributor.localId | A02604 | - |
dc.contributor.localId | A02709 | - |
dc.contributor.localId | A02813 | - |
dc.relation.journalcode | J00852 | - |
dc.identifier.eissn | 1399-3003 | - |
dc.identifier.pmid | 23314902 | - |
dc.identifier.url | http://erj.ersjournals.com/content/42/5/1379.long | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Cell Line | - |
dc.subject.keyword | Epithelial Cells/cytology* | - |
dc.subject.keyword | Epithelial-Mesenchymal Transition* | - |
dc.subject.keyword | Epithelium/metabolism* | - |
dc.subject.keyword | Gene Expression Profiling | - |
dc.subject.keyword | Gene Expression Regulation* | - |
dc.subject.keyword | Goblet Cells/metabolism | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Inflammation | - |
dc.subject.keyword | Mice | - |
dc.subject.keyword | Nasal Mucosa/metabolism | - |
dc.subject.keyword | Nasal Polyps/metabolism* | - |
dc.subject.keyword | Neuroendocrine Cells/cytology | - |
dc.subject.keyword | Phenotype | - |
dc.subject.keyword | Proprotein Convertase 1/physiology* | - |
dc.subject.keyword | Respiratory System/cytology | - |
dc.subject.keyword | Sinusitis/metabolism | - |
dc.subject.keyword | Wound Healing | - |
dc.contributor.alternativeName | Son, Myung Hyun | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.alternativeName | Lee, Da Hyung | - |
dc.contributor.alternativeName | Lee, Sang Nam | - |
dc.contributor.affiliatedAuthor | Son, Myung Hyun | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Lee, Da Hyung | - |
dc.contributor.affiliatedAuthor | Lee, Sang Nam | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 42 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1379 | - |
dc.citation.endPage | 1390 | - |
dc.identifier.bibliographicCitation | EUROPEAN RESPIRATORY JOURNAL, Vol.42(5) : 1379-1390, 2013 | - |
dc.identifier.rimsid | 32439 | - |
dc.type.rims | ART | - |
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