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중간엽 줄기세포의 골아세포 분화에 있어 STAT의 발현 및 역할

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dc.contributor.author이경미-
dc.contributor.author이진우-
dc.contributor.author정호선-
dc.contributor.author최윤락-
dc.contributor.author황지숙-
dc.date.accessioned2014-12-18T09:28:47Z-
dc.date.available2014-12-18T09:28:47Z-
dc.date.issued2013-
dc.identifier.issn1226-8933-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/88246-
dc.description.abstractPURPOSE: To investigate the role of STAT5 during osteogenesis differentiation of human mesenchymal stem cells(hMSC). MATERIALS AND METHODS: To assess the expression pattern of STATs during osteogenic differentiation, we performed western blot analysis and RT-PCR. By RNA interference, we have performed effect of STAT5A and STAT5B in osteogenesis. As a result of Luciferase assay, the promoter activity of DLX5 was decreased by STAT5A. RESULTS: To assess the expression pattern of STATs during osteogenic differentiation, we have performed western blot and RT-PCR after 14 day differentiation period. STAT1, 2, 3 and 4 showed no change in expression level during differentiation, while STAT5A and STAT5B displayed steady increase compared to the control. When STAT5A was knock down, the level of osteogenesis was increased. The transcriptional activity of DLX5 was decreased about 70% by STAT5A. CONCLUSION: The expression of STAT5A and STAT5B increased during osteogenesis of hMSC. Also, we shown that STAT5A regulated the transcriptional activity of DLX5. These results indicate that STAT5A acts as a pivotal transcription factor in osteogenesis of hMSC.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJournal of Korean Orthopaedic Research Society (대한정형외과연구학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title중간엽 줄기세포의 골아세포 분화에 있어 STAT의 발현 및 역할-
dc.title.alternativeDifferential Expression of STATs and Its Function in Osteogenesis of Mesenchymal Stem Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Orthopedic Surgery (정형외과학)-
dc.contributor.googleauthor황지숙-
dc.contributor.googleauthor이경미-
dc.contributor.googleauthor정호선-
dc.contributor.googleauthor이진우-
dc.contributor.googleauthor최윤락-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04619-
dc.contributor.localIdA03230-
dc.contributor.localIdA03785-
dc.contributor.localIdA04136-
dc.contributor.localIdA04489-
dc.relation.journalcodeJ01526-
dc.identifier.pmidMesenchymal stem cells ; Osteogenesis ; STAT5A ; STAT5B ; DLX5-
dc.subject.keywordMesenchymal stem cells-
dc.subject.keywordOsteogenesis-
dc.subject.keywordSTAT5A-
dc.subject.keywordSTAT5B-
dc.subject.keywordDLX5-
dc.contributor.alternativeNameLee, Kyung Mi-
dc.contributor.alternativeNameLee, Jin Woo-
dc.contributor.alternativeNameJung, Ho Sun-
dc.contributor.alternativeNameChoi, Yun Rak-
dc.contributor.alternativeNameHwang, Ji Suk-
dc.contributor.affiliatedAuthorLee, Kyoung Mi-
dc.contributor.affiliatedAuthorLee, Jin Woo-
dc.contributor.affiliatedAuthorJung, Ho Sun-
dc.contributor.affiliatedAuthorChoi, Yun Rak-
dc.contributor.affiliatedAuthorHwang, Ji Suk-
dc.rights.accessRightsfree-
dc.citation.volume16-
dc.citation.number1-
dc.citation.startPage1-
dc.citation.endPage9-
dc.identifier.bibliographicCitationJournal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.16(1) : 1-9, 2013-
dc.identifier.rimsid33153-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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