Emotion is closely related to the life of human, so has effect on many parts such as concentration, learning ability, etc. and makes to have different behavior patterns. The purpose of this paper is to extract important features based on physiological signals to recognize negative emotion. In this paper, after acquisition of electrocardiography(ECG), electroencephalography(EEG), skin temperature(SKT) and galvanic skin response(GSR) measurements based on physiological signals, we designed an accurate and fast algorithm using combination of linear discriminant analysis(LDA) and genetic algorithm(GA), then we selected important features. As a result, the accuracy of the algorithm is up to 96.4% and selected features are Mean, root mean square successive difference(RMSSD), NN intervals differing more than 50ms(NN50) of heart rate variability(HRV), σand α frequency power of EEG from frontal region, α, β, and γfrequency power of EEG from central region, and mean and standard deviation of SKT. Therefore, the features play an important role to recognize negative emotion.