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Induction of IL-6 and IL-8 Expression by Leptin Treatment in Periodontal Ligament Cells and Gingival Fibroblasts

 Hong-Gyu Park  ;  Ji-Hye Kim  ;  Jeong-Heon Cha  ;  Eun-Jung Bak  ;  Yun-Jung Yoo 
 International Journal of Oral Biology, Vol.38(2) : 73-80, 2013 
Journal Title
International Journal of Oral Biology
Issue Date
Periodontal ligament cells ; Gingival fibroblasts ; Leptin ; Interleukin-6 ; Interleukin-8
Leptin is one of the adipocytokines produced from adipose tissue but its functions in periodontal tissue have not previously been investigated. In our current study, we examined the effects of leptin on the expression of interleukin (IL)-6 and IL-8 in periodontal ligament (PDL) cells and gingival fibroblasts. Leptin receptor expression was evaluated by RT-PCR and the production of cytokines was measured by ELISA. The phosphorylation of Akt and Erk1/2 was assessed by western blotting. mRNA of long and short form leptin receptors were detected in both PDL cells and gingival fibroblasts. Leptin was found to increase the production of IL-6 and IL-8 in both of these cell types, an effect which was not blocked by polymyxin B, an inhibitor of lipopolysaccharide (LPS). Leptin did not alter the production of IL-6 and IL-8 induced by LPS in PDL cells but increased Akt and Erk1/2 phosphorylation in these cells. These results suggest that leptin acts as an inducer of IL-6 and IL-8 in PDL cells and gingival fibroblasts.
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2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ji Hye(김지혜)
Bak, Eun-Jung(박은정) ORCID logo https://orcid.org/0000-0002-7976-8594
Park, Hong Gyu(박홍규)
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Cha, Jeong Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
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