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Evaluation of the Bioefficacy of a Stabilized Form of Human Growth Hormone (SP-hGH)

Authors
 S.-B. Lee ; H. Park ; J. Kim ; H. Lee ; J. Koh 
Citation
 Hormone and Metabolic Research, Vol.45(10) : 722~727, 2013 
Journal Title
 Hormone and Metabolic Research 
ISSN
 0018-5043 
Issue Date
2013
Abstract
Protein aggregation is a major obstacle in maintaining the stability of therapeutic proteins. In previous studies, fusion between a stabilizing peptide (SP) and human growth hormone (hGH) resulted in improved solubility and stability compared with hGH alone, although the bioactivity of the protein was not confirmed in vivo. In this study, we evaluated the bioefficacy of hGH and SP-hGH in vivo using a mouse model. Subcutaneous injections of 30 μg of hGH or SP-hGH were administered to 8-month-old female mice, twice a week for 14 weeks. Neither hGH nor SP-hGH significantly affected body weight or blood glucose levels compared with control mice. Interestingly, abdominal fat was significantly reduced in SP-hGH-treated mice compared with hGH-treated mice. While total cholesterol, HDL, and LDL levels were slightly higher in both groups, TG levels were significantly reduced in both SP-hGH and hGH-treated mice compared with control mice. IGF-1 levels in the liver were increased in both the SP-hGH and hGH groups, thereby inducing liver cell proliferation. These results suggest that SP fusion with hGH attained similar or improved bioefficacy compared with hGH alone.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/88066
DOI
10.1055/s-0033-1345126
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Microbiology
Yonsei Authors
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Link
 https://www.thieme-connect.com/DOI/DOI?10.1055/s-0033-1345126
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