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The Antioxidative Effect of Heat-Shock Protein 70 in Dendritic Cells

Authors
 J. H. Je  ;  D. Y. Kim  ;  H. J. Roh  ;  C. Pak  ;  D. H. Kim  ;  D. Byamba  ;  H. Jee  ;  T.-G. Kim  ;  J. M. Park  ;  S.-K. Lee  ;  M.-G. Lee 
Citation
 SCANDINAVIAN JOURNAL OF IMMUNOLOGY, Vol.78(3) : 238-247, 2013 
Journal Title
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
ISSN
 0300-9475 
Issue Date
2013
MeSH
Animals ; Cell Line ; Dendritic Cells/immunology* ; Dendritic Cells/metabolism* ; Dermatitis, Contact/immunology ; Dermatitis, Contact/metabolism ; Enzyme Activation ; HSP70 Heat-Shock Proteins/metabolism* ; Haptens/immunology ; Interleukin-12/secretion ; Mice ; Mice, Inbred A ; Mice, Inbred BALB C ; Picryl Chloride/chemistry ; Protein Carbonylation* ; Reactive Oxygen Species/metabolism* ; Recombinant Fusion Proteins/metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Transfection ; Trinitrobenzenesulfonic Acid/immunology ; p38 Mitogen-Activated Protein Kinases/metabolism
Keywords
Animals ; Cell Line ; Dendritic Cells/immunology* ; Dendritic Cells/metabolism* ; Dermatitis, Contact/immunology ; Dermatitis, Contact/metabolism ; Enzyme Activation ; HSP70 Heat-Shock Proteins/metabolism* ; Haptens/immunology ; Interleukin-12/secretion ; Mice ; Mice, Inbred A ; Mice, Inbred BALB C ; Picryl Chloride/chemistry ; Protein Carbonylation* ; Reactive Oxygen Species/metabolism* ; Recombinant Fusion Proteins/metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Transfection ; Trinitrobenzenesulfonic Acid/immunology ; p38 Mitogen-Activated Protein Kinases/metabolism
Abstract
Reactive oxygen species (ROS) are produced by dendritic cells (DCs) during antigen presentation in contact hypersensitivity (CHS). ROS cause a number of non-enzymatic protein modifications, such as carbonylation. Carbonylated proteins in DCs in response to hapten have not been fully identified yet. To identify the proteins carbonylated by ROS, murine epidermis-derived DC line XS106 was challenged with a hapten, 2,4,6-trinitrobenzene sulphonic acid (TNBS). MALDI-TOF analysis revealed that heat-shock protein 70 (HSP70) was one of the carbonylated proteins induced by TNBS. To verify the role of HSP70 in TNBS-treated XS106 cell, we fused protein transduction domain (PTD) with HSP70 to facilitate protein delivery into the cell. The transfected fusion protein HSP70 within the cell caused transient increase of the cellular level of HSP70. Transient increase of HSP70 level in XS-106 DCs resulted in inhibition of ROS production, carbonylation of HSP70, p38 MAPK activation and subsequently IL-12 secretion. To investigate the effects of PTD–HSP70 in vivo, ear-swelling experiments with 2,4,6-trinitro-1-chlorobenzene (TNCB) were performed in BALB/c mice. Pretreatment of PTD–HSP70 reduced the CHS response to TNCB in vivo. We report here that carbonylation of HSP70 by ROS is associated with the pathogenesis of CHS, suggesting possibility of HSP70-targeting therapy in CHS.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/sji.12078/abstract
DOI
10.1111/sji.12078
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Do Young(김도영)
Kim, Dong Hyun(김동현)
Kim, Tae-Gyun(김태균) ORCID logo https://orcid.org/0000-0002-2116-4579
Roh, Hyo Jin(노효진)
Lee, Min Geol(이민걸) ORCID logo https://orcid.org/0000-0001-7040-5335
Je, Jeong Hwan(제정환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87718
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