3 324

Cited 14 times in

Differential effects of topical corticosteroid and calcineurin inhibitor on the epidermal tight junction

Authors
 Sang E. Lee  ;  Yuri Choi  ;  Song-Ee Kim  ;  Eun B. Noh  ;  Soo-Chan Kim 
Citation
 EXPERIMENTAL DERMATOLOGY, Vol.22(1) : 59-61, 2013 
Journal Title
EXPERIMENTAL DERMATOLOGY
ISSN
 0906-6705 
Issue Date
2013
MeSH
Administration, Cutaneous ; Animals ; Calcineurin Inhibitors ; Claudin-1/genetics ; Claudin-1/metabolism ; Claudin-4/genetics ; Claudin-4/metabolism ; Clobetasol/pharmacology* ; Dermatologic Agents/pharmacology ; Gene Expression ; Glucocorticoids/pharmacology ; Mice ; Occludin/genetics ; Occludin/metabolism ; Permeability/drug effects ; RNA, Messenger/metabolism ; Skin/drug effects* ; Tacrolimus/pharmacology* ; Tight Junctions/drug effects* ; Tight Junctions/metabolism* ; Tight Junctions/pathology ; Water Loss, Insensible/drug effects
Keywords
Administration, Cutaneous ; Animals ; Calcineurin Inhibitors ; Claudin-1/genetics ; Claudin-1/metabolism ; Claudin-4/genetics ; Claudin-4/metabolism ; Clobetasol/pharmacology* ; Dermatologic Agents/pharmacology ; Gene Expression ; Glucocorticoids/pharmacology ; Mice ; Occludin/genetics ; Occludin/metabolism ; Permeability/drug effects ; RNA, Messenger/metabolism ; Skin/drug effects* ; Tacrolimus/pharmacology* ; Tight Junctions/drug effects* ; Tight Junctions/metabolism* ; Tight Junctions/pathology ; Water Loss, Insensible/drug effects
Abstract
Tight junction (TJ) is one of the functional barriers present in the skin. Although topical corticosteroids and calcineurin inhibitors are used widely for atopic dermatitis, the effect of these agents on TJs has not been reported. We investigated the structural changes of TJs in mice skin after application of 0.05% clobetasol propionate or 0.1% tacrolimus ointment for 10 days. Clobetasol caused epidermal thinning and decreased collagen density. Basal transepidermal water loss was significantly increased in clobetasol-treated versus vehicle- or tacrolimus-treated skin. Confocal immunofluorescence showed that clobetasol altered the structure of claudin-1,-4 and occludin. Tacrolimus also caused morphological alteration of occludin. Western blot and real-time PCR revealed that clobetasol significantly decreased claudin-1,-4 and occludin, whereas tacrolimus did not significantly affect claudin-1 and -4 but downregulated occludin to a lesser extent compared to clobetasol. In conclusion, we suggest that downregulation of TJ proteins expression is another pathomechanism of corticosteroid-induced permeability barrier disruption.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/exd.12055/abstract
DOI
10.1111/exd.12055
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Song Ee(김송이)
Kim, Soo Chan(김수찬) ORCID logo https://orcid.org/0000-0002-2327-4755
Lee, Sang Eun(이상은) ORCID logo https://orcid.org/0000-0003-4720-9955
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87435
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links