10 288

Cited 6 times in

Both the sera of patients with Behçet’s disease and Streptococcus sanguis stimulate membrane expression of hnRNP A2/B1 in endothelial cells

Authors
 SB Cho  ;  Z Zheng  ;  S Cho  ;  KJ Ahn  ;  MJ Choi  ;  D-Y Kim  ;  KH Lee  ;  D Bang 
Citation
 Scandinavian Journal of Rheumatology, Vol.42(3) : 241-246, 2013 
Journal Title
 Scandinavian Journal of Rheumatology 
ISSN
 0300-9742 
Issue Date
2013
MeSH
Behcet Syndrome/immunology* ; Case-Control Studies ; Cell Membrane/metabolism ; Cells, Cultured ; Endothelial Cells/metabolism* ; Fluorescent Antibody Technique, Direct ; Heterogeneous-Nuclear Ribonucleoprotein Group A-B/metabolism* ; Humans ; Streptococcus sanguis/immunology*
Keywords
Behcet Syndrome/immunology* ; Case-Control Studies ; Cell Membrane/metabolism ; Cells, Cultured ; Endothelial Cells/metabolism* ; Fluorescent Antibody Technique, Direct ; Heterogeneous-Nuclear Ribonucleoprotein Group A-B/metabolism* ; Humans ; Streptococcus sanguis/immunology*
Abstract
Objectives: Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 has been identified as a target antigen of anti-endothelial cell immunglobulin (Ig)A antibodies in patients with Behçet’s disease (BD). The aim was to investigate the effects of the sera from BD patients and Streptococcus sanguis on the subcellular expression of hnRNP A2/B1 in human dermal microvascular endothelial cells (HDMECs). Method: The sera of BD patients and healthy controls (HC) as well as cultured S. sanguis were used to stimulate HDMECs. Subcellular fractions were obtained from stimulated HDMECs and were subjected to immunoblot analyses. The distribution of hnRNP A2/B1 was investigated by immunocytochemistry and direct immunofluorescence study was performed in biopsy specimens of mucosal ulcers from BD patients. Results: BD patients’ sera increased the membrane expression of hnRNP A2/B1 in HDMECs after 12 and 24 h of incubation compared with HDMECs incubated with endothelial cell culture media and HC sera. S. sanguis also increased hnRNP A2/B1 in the cellular membrane. hnRNP A2/B1 mRNA level was also significantly upregulated in HDMECs incubated with BD patients’ sera and S. sanguis. Immunocytochemistry demonstrated marked expression of hnRNP A2/B1 in the cytoplasm and cellular membrane of HDMECs incubated with BD patients’ sera or S. sanguis. In addition, direct immunofluorescence experiments revealed the co-localization of serum IgA antibodies and monoclonal antibodies (mAbs) against hnRNP A2/B1 in tissue sections from ulcers of BD patients. Conclusions: Our data indicate that both the sera of BD patients with active disease and S. sanguis infection are inflammatory stimuli that can induce membranous hnRNP A2/B1 expression in HDMECs.
Full Text
http://informahealthcare.com/doi/abs/10.3109/03009742.2012.733728
DOI
10.3109/03009742.2012.733728
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Do Young(김도영)
Bang, Dong Sik(방동식)
Lee, Kwang Hoon(이광훈)
Zheng, Zhen Long(정진룡)
Cho, Sung Bin(조성빈)
Cho, Su Hyun(조수현)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87340
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse