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Rk1, a Ginsenoside, Is a New Blocker of Vascular Leakage Acting through Actin Structure Remodeling.

Authors
 Yong-Sun Maeng  ;  Sony Maharjan  ;  Jeong-Hun Kim  ;  Jeong-Hill Park  ;  Young Suk Yu  ;  Young-Myoung Kim  ;  Young-Guen Kwon 
Citation
 PLOS ONE, Vol.8(7) : e68659, 2013 
Journal Title
PLOS ONE
Issue Date
2013
MeSH
Actins/chemistry* ; Actins/metabolism* ; Animals ; Blood Vessels/cytology ; Blood Vessels/drug effects* ; Blood Vessels/metabolism* ; Cortactin/metabolism ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Ginsenosides/pharmacology* ; Glycation End Products, Advanced/pharmacology ; Histamine/pharmacology ; Humans ; Male ; Mice ; Myosin Light Chains/metabolism ; Permeability/drug effects ; Phosphorylation/drug effects ; Retina/cytology ; Thrombin/pharmacology ; Tight Junctions/drug effects ; Tight Junctions/metabolism ; Vascular Endothelial Growth Factor A/pharmacology
Keywords
Actins/chemistry* ; Actins/metabolism* ; Animals ; Blood Vessels/cytology ; Blood Vessels/drug effects* ; Blood Vessels/metabolism* ; Cortactin/metabolism ; Endothelial Cells/drug effects ; Endothelial Cells/metabolism ; Ginsenosides/pharmacology* ; Glycation End Products, Advanced/pharmacology ; Histamine/pharmacology ; Humans ; Male ; Mice ; Myosin Light Chains/metabolism ; Permeability/drug effects ; Phosphorylation/drug effects ; Retina/cytology ; Thrombin/pharmacology ; Tight Junctions/drug effects ; Tight Junctions/metabolism ; Vascular Endothelial Growth Factor A/pharmacology
Abstract
Endothelial barrier integrity is essential for vascular homeostasis and increased vascular permeability and has been implicated in many pathological processes, including diabetic retinopathy. Here, we investigated the effect of Rk1, a ginsenoside extracted from sun ginseng, on regulation of endothelial barrier function. In human retinal endothelial cells, Rk1 strongly inhibited permeability induced by VEGF, advanced glycation end-product, thrombin, or histamine. Furthermore, Rk1 significantly reduced the vessel leakiness of retina in a diabetic mouse model. This anti-permeability activity of Rk1 is correlated with enhanced stability and positioning of tight junction proteins at the boundary between cells. Signaling experiments revealed that Rk1 induces phosphorylation of myosin light chain and cortactin, which are critical regulators for the formation of the cortical actin ring structure and endothelial barrier. These findings raise the possibility that ginsenoside Rk1 could be exploited as a novel prototype compound for the prevention of human diseases that are characterized by vascular leakage.
Files in This Item:
T201302273.pdf Download
DOI
10.1371/journal.pone.0068659
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
Yonsei Authors
Maeng, Yong Sun(맹용선) ORCID logo https://orcid.org/0000-0003-1694-8405
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87236
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