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E-beam fabrication of antibacterial silver nanoparticles on diameter-controlled TiO2 nanotubes for bio-implants

Authors
 Soo-Hyuk Uhm  ;  Doo-Hoon Song  ;  Jae-Sung Kwon  ;  Sang-Bae Lee  ;  Jeon-Geon Han  ;  Kwang-Mahn Kim  ;  Kyoung-Nam Kim 
Citation
 SURFACE & COATINGS TECHNOLOGY, Vol.228(1) : 360-366, 2013 
Journal Title
 SURFACE & COATINGS TECHNOLOGY 
ISSN
 0257-8972 
Issue Date
2013
Keywords
TiO2 nanotube ; Antibacterial ; Anodization ; E-beam evaporation ; Silver nanoparticle
Abstract
To reduce the risk of infection, many researchers have applied antibacterial silver to TiO2 implant surfaces. In this study, silver nanoparticles were applied to TiO2 nanotubes with e-beam evaporation. In advance, the diameters of the nanotubes were controlled by varying anodization. The objective of this study was to optimize the antibacterial effects of silver nanoparticles, but also maintain the nanotubular surface of biocompatible TiO2 nanotubes. The surfaces were characterized with field emission scanning electron microscopy (FE-SEM), energy dispersive spectroscopy (EDS), high resolution X-ray diffraction (HR-XRD), X-ray photoelectron spectroscopy (XPS) and scanning probe microscopy (SPM). Contact angle measurements and 3 times simulated body fluid immersion (SBF) tests were performed for indirect verification of osteogenic properties. Results showed that the silver nanoparticles were efficiently fabricated on the nanotubes. We found that the contact angle was influenced more by the degree of anatase phase in the TiO2 than by surface roughness and apatite forming ability was proportionally increased as hydrophilicity was increased. Silver ion release was measured by the inductively coupled plasma mass spectrometry (ICP-MS). All specimens showed more than 0.1 ppb of silver concentration which was known to be the level for the antibacterial property. The antibacterial activity test against Staphylococcus aureus (S. aureus) showed that bacterial colonization was effectively inhibited in all experimental groups. Water soluble tetrazolium (WST) assays showed that silver nanoparticles fabricated on large-diameter nanotubes were not cytotoxic. However, smaller diameter of nanotubes showed mild cytotoxicity, due to the excess of silver nanoparticles aggregated on the top surface. Overall, we concluded that large-diameter TiO2 nanotubes had favorable osteogenic properties, were non-cytotoxic, maintained a nanoporous surface, and displayed antibacterial properties that were highly beneficial for bio-implants.
Full Text
http://www.sciencedirect.com/science/article/pii/S0257897212005178
DOI
10.1016/j.surfcoat.2012.05.102
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Research Center for Orofacial Hard Tissue Regeneration (구강악안면경조직재생연구센터) > 1. Journal Papers
Yonsei Authors
Kwon, Jae-Sung(권재성) ORCID logo https://orcid.org/0000-0001-9803-7730
Kim, Kyoung Nam(김경남)
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Song, Doo Hoon(송두훈)
Uhm, Soo Hyuk(엄수혁)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87147
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