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A microfluidic array for quantitative analysis of human neural stem cell self-renewal and differentiation in three-dimensional hypoxic microenvironment

Authors
 Kisuk Yang  ;  Sewoon Han  ;  Yoojin Shin  ;  Eunkyung Ko  ;  Jin Kim  ;  Kook In Park  ;  Seok Chung  ;  Seung-Woo Cho 
Citation
 BIOMATERIALS, Vol.34(28) : 6607-6614, 2013 
Journal Title
BIOMATERIALS
ISSN
 0142-9612 
Issue Date
2013
MeSH
Cell Culture Techniques ; Cell Differentiation/physiology ; Cell Hypoxia/physiology ; Cells, Cultured ; Humans ; Microfluidics/methods* ; Models, Biological ; Neural Stem Cells/cytology*
Keywords
Microfluidic array ; Neural stem cells ; Hypoxia ; Self-renewal ; Differentiation
Abstract
We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an in vivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.
Full Text
http://www.sciencedirect.com/science/article/pii/S0142961213006595
DOI
10.1016/j.biomaterials.2013.05.067
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
Yonsei Authors
Park, Kook In(박국인) ORCID logo https://orcid.org/0000-0001-8499-9293
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87097
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