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Telomerase- and angiogenesis-related gene responses to irradiation in human umbilical vein endothelial cells

Authors
 Hyun Chang  ;  Sun Young Rha  ;  Hei-Cheul Jeung  ;  Kyu Hyun Park  ;  Tae Soo Kim  ;  Yong-Bae Kim  ;  Hyun Cheol Chung 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, Vol.31(5) : 1202-1208, 2013 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 
ISSN
 1107-3756 
Issue Date
2013
MeSH
Cellular Senescence/genetics ; Cellular Senescence/radiation effects ; Gene Expression Regulation/radiation effects* ; Human Umbilical Vein Endothelial Cells/enzymology ; Human Umbilical Vein Endothelial Cells/metabolism* ; Human Umbilical Vein Endothelial Cells/radiation effects* ; Humans ; Neovascularization, Physiologic/genetics* ; Neovascularization, Physiologic/radiation effects* ; Phenotype ; Radiation, Ionizing* ; Telomerase/metabolism*
Keywords
Cellular Senescence/genetics ; Cellular Senescence/radiation effects ; Gene Expression Regulation/radiation effects* ; Human Umbilical Vein Endothelial Cells/enzymology ; Human Umbilical Vein Endothelial Cells/metabolism* ; Human Umbilical Vein Endothelial Cells/radiation effects* ; Humans ; Neovascularization, Physiologic/genetics* ; Neovascularization, Physiologic/radiation effects* ; Phenotype ; Radiation, Ionizing* ; Telomerase/metabolism*
Abstract
In this study, we investigated the effects of ionizing radiation (IR) on human umbilical vein endothelial cells (HUVECs) in the context of senescence. HUVECs at passage number (PN)1, PN2 and PN3 were exposed to irradiation (2 Gy). The growth rate of the HUVECS was measured by proliferation assay and senescence-associated β-galactosidase assay was used to measure the number of senescent cells. Telomerase activity and the expression of telomerase- and angiogenesis-related genes were measured by telomerase assay and real-time PCR, respectively. The number of senescent cells was significantly increased in the irradiated HUVECs at all PNs. Compared to the controls, telomerase activity, the expression of human telomerase reverse transcriptase (hTERT) and c-Myc in the irradiated HUVECs were downregulated during serial passage. The downregulation of vascular endothelial growth factor (VEGF) was observed in the irradiated HUVECs as the PN increased. The data presented in this study may aid in the understanding of the mechanisms behind IR‑induced EC senescence and telomerase- and angiogenesis‑related gene response.
Full Text
http://www.spandidos-publications.com/ijmm/31/5/1202
DOI
10.3892/ijmm.2013.1300
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yong Bae(김용배) ORCID logo https://orcid.org/0000-0001-7573-6862
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
Jeung, Hei Cheul(정희철) ORCID logo https://orcid.org/0000-0003-0952-3679
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86665
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