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Xanthorrhizol Induces Apoptosis Through ROS-Mediated MAPK Activation in Human Oral Squamous Cell Carcinoma Cells and Inhibits DMBA-Induced Oral Carcinogenesis in Hamsters

 Ju Yeon Kim  ;  Jeong Mi An  ;  Won-Yoon Chung  ;  Kwang-Kyun Park  ;  Jae Kwan Hwang  ;  Du Sik Kim  ;  Su Ryeon Seo  ;  Jeong Taeg Seo 
 PHYTOTHERAPY RESEARCH, Vol.27(4) : 493-498, 2013 
Journal Title
Issue Date
9,10-Dimethyl-1,2-benzanthracene ; Animals ; Antineoplastic Agents, Phytogenic/pharmacology ; Apoptosis/drug effects* ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/pathology* ; Caspase Inhibitors/pharmacology ; Cell Line, Tumor ; Cell Survival ; Cricetinae ; Humans ; MAP Kinase Signaling System/drug effects* ; Male ; Mouth Neoplasms/metabolism ; Mouth Neoplasms/pathology* ; Neoplasms, Experimental/metabolism ; Neoplasms, Experimental/pathology ; Phenols/pharmacology* ; Reactive Oxygen Species/metabolism
oral squamous cell carcinoma cells ; xanthorrhizol ; PARP ; caspases ; ROS ; MAPKs
Xanthorrhizol, a natural sesquiterpenoid compound isolated from Curcuma xanthorrhiza Roxb, has been known to inhibit the growth of human colon, breast, liver and cervical cancer cells. In this study, xanthorrhizol decreased cell viability, induced apoptosis and decreased the level of full-length PARP in SCC-15 oral squamous cell carcinoma (OSCC) cells. A decrease in cell viability and PARP degradation was not prevented by treatment with the caspase inhibitor Z-VAD-fmk in xanthorrhizol-treated cells. Xanthorrhizol treatment elevated intracellular Ca2+ and ROS levels in SCC-15 cells. Treatment with a Ca2+ chelator, EGTA/AM, did not affect xanthorrhizol- induced cytotoxicity, but cell viability was partly recovered by treatment with endogenous antioxidant, GSH, or hydroxy radical trapper, MCI-186. Furthermore, the viability of xanthorrhizol-treated SCC-15 cells was significantly restored by treatment with SB203580 and/or SP600125 but not significantly by PD98059 treatment. Xanthorrhizol-induced activation of p38 MAPK and JNK was blocked by MCI-186. Finally, xanthorrhizol suppressed the number of tumors in buccal pouches and increased the survival rate in hamsters treated with 7,12-dimethylbenz[a]anthracene. In conclusion, xanthorrhizol may induce caspase-independent apoptosis through ROS-mediated p38 MAPK and JNK activation in SCC-15 OSCC cells and prevent chemical-induced oral carcinogenesis. Therefore, xanthorrhizol seems to be a promising chemopreventive agent.
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2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Kwang Kyun(박광균)
Seo, Jeong Taeg(서정택) ORCID logo https://orcid.org/0000-0003-2697-0251
Chung, Won Yoon(정원윤) ORCID logo https://orcid.org/0000-0001-8428-9005
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