4 85

Cited 2 times in

Streptococcus sanguinis and the sera of patients with Behçet’s disease stimulate membrane expression of α-enolase in human dermal microvascular endothelial cells

 Suhyun Cho ; Zhenlong Zheng ; Dongsik Bang ; Kwang Hoon Lee ; Min Ju Choi ; Sung Bin Cho 
 Archives of Dermatological Research, Vol.305(3) : 223~232, 2013 
Journal Title
 Archives of Dermatological Research 
Issue Date
The glycolytic enzyme α-enolase is a plasminogen-binding protein that is generally found in the cytosolic compartment. However, α-enolase can also be expressed on cell surfaces following an inflammatory stimulus via an unknown mechanism. We investigated the effects of Streptococcus sanguinis (S. sanguinis) and the sera of patients with Behçet's disease (BD) on theexpression and distribution of α-enolase in human dermal microvascular endothelial cells (HDMECs). HDMECs were stimulated with cultured S. sanguinis and the sera of active BD patients. HDMECs incubated for 6, 12 or 24 h were harvested, and themembrane and cytoplasmic fractions of proteins were extracted. The expression and distribution of α-enolase were analyzed using subcellular fractionation and immunoblotting. Subcellular localization of α-enolase was also assessed by immunocytochemistry. S. sanguinis stimulated the expression of α-enolase in the membranous compartment of HDMECs in a dose-dependent manner. This pattern was also observed in HDMECs incubated with BD patients' sera. Although incubation of HDMECs with sera from healthy controls increased membrane expression of α-enolase, incubation with BD sera resulted in earlier and higher expression of this glycoprotein in the cellular membrane of HDMECs. Immunocytochemistry revealed strong immunostaining of α-enolase in the cytoplasm and cytoplasmic membrane of HDMECs incubated with S. sanguinis or BDpatients' sera. In conclusions, these results indicate that S. sanguinis infection and the sera of BD patients with active diseaseare inflammatory stimuli that can induce membranous α-enolase expression in endothelial cells. Membrane-expressed α-enolase could potentially react with anti-α-enolase antibodies in BD patients' sera, resulting in increased inflammation.
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Yonsei Biomedical Research Center
1. 연구논문 > 1. College of Medicine > Dept. of Dermatology
Yonsei Authors
사서에게 알리기
RIS (EndNote)
XLS (Excel)


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.