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Increased Alternative Lengthening of Telomere Phenotypes of Telomerase-negative Immortal Cells upon Trichostatin – A Treatment

Authors
 A RA JUNG ; JEONG EUN YOO ; BONG-KYEONG OH ; SUN YOUNG RHA ; HYUN CHEOL CHUNG ; HEI-CHEUL JEUNG ; YE-NA CHOI ; HONG-HEE SHIM 
Citation
 Anticancer Research, Vol.33(3) : 821~830, 2013 
Journal Title
 Anticancer Research 
ISSN
 0250-7005 
Issue Date
2013
Abstract
Human immortal cells maintain their telomeres either by telomerase or by alternative lengthening of telomeres (ALT) that is based on homologous telomeric recombination. Previous studies showed that the ALT mechanism is activated in non-ALT cells when heterochromatic features are reduced. In this study, we examined the ALT phenotypes of ALT cells after treatment with trichostatin-A (TSA), which is an inhibitor of histone deacetylases and causes global chromatin decondensation. The ALT cells remained telomerase-negative after TSA treatment. ALT-associated promyelocytic leukemia (PML) nuclear bodies and telomere sister chromatid exchanges, typical ALT phenotypes, markedly increased in the TSA-treated cells, while the telomere length remained unchanged. In addition, telomerase expression in the ALT cells suppressed TSA-mediated ALT phenotype enhancement. Our results show that certain ALT phenotypes become more pronounced when chromatin is decondensed, and also suggest that the ALT mechanism may compete with telomerase for telomere maintenance in cells that lack heterochromatin.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/86536
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Pathology
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
1. 연구논문 > 1. College of Medicine > Medical Research Center
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Link
 http://ar.iiarjournals.org/content/33/3/821.long
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