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Serum levels of anti-type VII collagen antibodies detected by enzyme-linked immunosorbent assay in patients with epidermolysis bullosa acquisita are correlated with the severity of skin lesions

Authors
 J.H. Kim  ;  Y.H. Kim  ;  S. Kim  ;  E.B. Noh  ;  S-E. Kim  ;  A. Vorobyev  ;  E. Schmidt  ;  D. Zillikens  ;  S-C. Kim 
Citation
 Journal of the European Academy of Dermatology and Venereology, Vol.27(2) : 224-230, 2013 
Journal Title
 Journal of the European Academy of Dermatology and Venereology 
ISSN
 0926-9959 
Issue Date
2013
Abstract
BACKGROUND: Epidermolysis bullosa acquisita (EBA) is a chronic autoimmune subepidermal bullous disease characterized by circulating autoantibodies against type VII collagen. Detecting these autoantibodies is crucial for the diagnosis of this disease, and is also useful for measuring disease activity. Enzyme-linked immunosorbent assay (ELISA), a quantitative method to measure anti-type VII collagen antibody levels, is currently available to diagnose EBA. OBJECTIVE: The aim of this study was to investigate the relationship of ELISA with overall clinical severity. METHODS: Sera from patients with EBA (n = 30), bullous pemphigoid (n = 20), anti-laminin γ1 pemphigoid (n = 9) and healthy donors (n = 24) were tested using ELISA, using the recombinant non-collagenous 1 (NC1) and 2 (NC2) domains of type VII collagen. Relationships between clinical characteristics, indirect immunofluoroscence (IIF) titres and ELISA values were investigated. RESULTS: The sensitivity and specificity of the EBA ELISA were 96.7% and 98.1%, respectively. There was no significant difference between ELISA results for classic and inflammatory types. The severity of skin involvement was positively correlated with both ELISA value (r = 0.87, P < 0.01) and IIF titre (r = 0.59, P < 0.01). Time sequence analysis in four patients with EBA showed that ELISA values reflect disease activity better than IIF titres. CONCLUSIONS: Type VII collagen ELISA using the NC1 and NC2 domains is useful for diagnosing EBA and monitoring disease severity.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86458
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1468-3083.2012.04617.x/abstract
DOI
10.1111/j.1468-3083.2012.04617.x
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실)
Yonsei Authors
김송이(Kim, Song Ee)
김수찬(Kim, Soo Chan)
김종훈(Kim, Jong Hoon) ORCID logo https://orcid.org/0000-0002-3385-8180
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