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DNA methylation-specific multiplex assays for body fluid identification

 Ja Hyun An  ;  Ajin Choi  ;  Kyoung-Jin Shin  ;  Woo Ick Yang  ;  Hwan Young Lee 
 International Journal of Legal Medicine, Vol.127(1) : 35-43, 2013 
Journal Title
 International Journal of Legal Medicine 
Issue Date
Recent advances in whole-genome epigenetic analysis indicate that chromosome segments called tissue-specific differentially methylated regions (tDMRs) show different DNA methylation profiles according to cell or tissue type. Therefore, body fluid-specific differential DNA methylation is a promising indicator for body fluid identification. However, DNA methylation patterns are susceptible to change in response to environmental factors and aging. Therefore, we investigated age-related methylation changes in semen-specific tDMRs using body fluids from young and elderly men. After confirming the stability of the body fluid-specific DNA methylation profile over time, two different multiplex PCR systems were constructed using methylation-sensitive restriction enzyme PCR and methylation SNaPshot, in order to analyze the methylation status of specific CpG sites from the USP49, DACT1, PRMT2, and PFN3 tDMRs. Both multiplex systems could successfully identify semen with spermatozoa and could differentiate menstrual blood and vaginal fluids from blood and saliva. Although including more markers for body fluid identification might be necessary, this study adds to the support that body fluid identification by DNA methylation profiles could be a valuable tool for forensic analysis of body fluids.
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Forensic Medicine (법의학과)
Yonsei Authors
신경진(Shin, Kyoung Jin)
안자현(An, Ja Hyun)
양우익(Yang, Woo Ick)
이환영(Lee, Hwan Young)
최아진(Choi, A Jin)
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