Cited 8 times in
Alpha B-crystallin prevents the arrhythmogenic effects of particulate matter isolated from ambient air by attenuating oxidative stress
DC Field | Value | Language |
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dc.contributor.author | 정지형 | - |
dc.contributor.author | 김창수 | - |
dc.contributor.author | 황기철 | - |
dc.contributor.author | 박혜림 | - |
dc.contributor.author | 박희남 | - |
dc.contributor.author | 송병욱 | - |
dc.contributor.author | 이문형 | - |
dc.contributor.author | 전현주 | - |
dc.contributor.author | 정보영 | - |
dc.date.accessioned | 2014-12-18T08:24:53Z | - |
dc.date.available | 2014-12-18T08:24:53Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0041-008X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/86261 | - |
dc.description.abstract | Ca2 +/calmodulin-dependent protein kinase II (CaMKII) is activated by particulate matter (PM) isolated from ambient air and linked to prolonged repolarization and cardiac arrhythmia. We evaluated whether alpha B-crystallin (CryAB), a heat shock protein, could prevent the arrhythmogenic effects of PM by preventing CaMKII activation. CryAB was delivered into cardiac cells using a TAT-protein transduction domain (TAT-CryAB). ECGs were measured before and after tracheal exposure of diesel exhaust particles (DEP) and each intervention in adult Sprague–Dawley rats. After endotracheal exposure of DEP (200 μg/mL for 30 minutes, n = 11), QT intervals were prolonged from 115 ± 14 ms to 144 ± 20 ms (p = 0.03), and premature ventricular contractions were observed more frequently (0% vs. 44%) than control (n = 5) and TAT-Cry (n = 5). However, DEP-induced arrhythmia was not observed in TAT-CryAB (1 mg/kg) pretreated rats (n = 5). In optical mapping of Langendorff-perfused rat heats, compared with baseline, DEP infusion of 12.5 μg/mL (n = 12) increased apicobasal action potential duration (APD) differences from 2 ± 6 ms to 36 ± 15 ms (p < 0.001), APD restitution slope from 0.26 ± 0.07 to 1.19 ± 0.11 (p < 0.001) and ventricular tachycardia (VT) from 0% to 75% (p < 0.001). DEP infusion easily induced spatially discordant alternans. However, the effects of DEP were prevented by TAT-CryAB (1 mg/kg, n = 9). In rat myocytes, while DEP increased reactive oxygen species (ROS) generation and phosphated CaMKII, TAT-CryAB prevented these effects. In conclusion, CryAB, a small heat shock protein, might prevent the arrhythmogenic effects of PM by attenuating ROS generation and CaMKII activation. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | TOXICOLOGY AND APPLIED PHARMACOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Action Potentials | - |
dc.subject.MESH | Air Pollutants/toxicity* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Arrhythmias, Cardiac/chemically induced | - |
dc.subject.MESH | Arrhythmias, Cardiac/prevention & control* | - |
dc.subject.MESH | Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Myocytes, Cardiac/metabolism | - |
dc.subject.MESH | Oxidative Stress/physiology* | - |
dc.subject.MESH | Particulate Matter/toxicity* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism | - |
dc.subject.MESH | Vehicle Emissions/toxicity | - |
dc.subject.MESH | Ventricular Premature Complexes/chemically induced | - |
dc.subject.MESH | Ventricular Premature Complexes/prevention & control | - |
dc.subject.MESH | alpha-Crystallin B Chain/administration & dosage | - |
dc.subject.MESH | alpha-Crystallin B Chain/physiology* | - |
dc.title | Alpha B-crystallin prevents the arrhythmogenic effects of particulate matter isolated from ambient air by attenuating oxidative stress | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Preventive Medicine (예방의학) | - |
dc.contributor.googleauthor | Hyelim Park | - |
dc.contributor.googleauthor | Sanghoon Park | - |
dc.contributor.googleauthor | Hyunju Jeon | - |
dc.contributor.googleauthor | Byeong-Wook Song | - |
dc.contributor.googleauthor | Jin-Bae Kim | - |
dc.contributor.googleauthor | Chang-Soo Kim | - |
dc.contributor.googleauthor | Hui-Nam Pak | - |
dc.contributor.googleauthor | Ki-Chul Hwang | - |
dc.contributor.googleauthor | Moon-Hyoung Lee | - |
dc.contributor.googleauthor | Ji Hyung Chung | - |
dc.contributor.googleauthor | Boyoung Joung | - |
dc.identifier.doi | 10.1016/j.taap.2012.10.013 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03739 | - |
dc.contributor.localId | A01042 | - |
dc.contributor.localId | A04456 | - |
dc.contributor.localId | A01760 | - |
dc.contributor.localId | A01776 | - |
dc.contributor.localId | A02026 | - |
dc.contributor.localId | A02766 | - |
dc.contributor.localId | A03562 | - |
dc.contributor.localId | A03609 | - |
dc.relation.journalcode | J02742 | - |
dc.identifier.eissn | 1096-0333 | - |
dc.identifier.pmid | 23153557 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0041008X12004553 | - |
dc.subject.keyword | Particulate matter | - |
dc.subject.keyword | Heat shock protein | - |
dc.subject.keyword | Air pollution | - |
dc.subject.keyword | Arrhythmia | - |
dc.subject.keyword | Ca2 +/calmodulin-dependent protein kinase II | - |
dc.contributor.alternativeName | Chung, Ji Hyung | - |
dc.contributor.alternativeName | Kim, Chang Soo | - |
dc.contributor.alternativeName | Hwang, Ki Chul | - |
dc.contributor.alternativeName | Park, Hye Lim | - |
dc.contributor.alternativeName | Pak, Hui Nam | - |
dc.contributor.alternativeName | Song, Byeong Wook | - |
dc.contributor.alternativeName | Lee, Moon Hyoung | - |
dc.contributor.alternativeName | Jeon, Hyun Ju | - |
dc.contributor.alternativeName | Joung, Bo Young | - |
dc.contributor.affiliatedAuthor | Chung, Ji Hyung | - |
dc.contributor.affiliatedAuthor | Kim, Chang Soo | - |
dc.contributor.affiliatedAuthor | Hwang, Ki Chul | - |
dc.contributor.affiliatedAuthor | Park, Hye Lim | - |
dc.contributor.affiliatedAuthor | Pak, Hui Nam | - |
dc.contributor.affiliatedAuthor | Song, Byeong Wook | - |
dc.contributor.affiliatedAuthor | Lee, Moon Hyoung | - |
dc.contributor.affiliatedAuthor | Jeon, Hyun Ju | - |
dc.contributor.affiliatedAuthor | Joung, Bo Young | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 266 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 267 | - |
dc.citation.endPage | 275 | - |
dc.identifier.bibliographicCitation | TOXICOLOGY AND APPLIED PHARMACOLOGY, Vol.266(2) : 267-275, 2013 | - |
dc.identifier.rimsid | 28877 | - |
dc.type.rims | ART | - |
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