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Alpha B-crystallin prevents the arrhythmogenic effects of particulate matter isolated from ambient air by attenuating oxidative stress

DC Field Value Language
dc.contributor.author정지형-
dc.contributor.author김창수-
dc.contributor.author황기철-
dc.contributor.author박혜림-
dc.contributor.author박희남-
dc.contributor.author송병욱-
dc.contributor.author이문형-
dc.contributor.author전현주-
dc.contributor.author정보영-
dc.date.accessioned2014-12-18T08:24:53Z-
dc.date.available2014-12-18T08:24:53Z-
dc.date.issued2013-
dc.identifier.issn0041-008X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/86261-
dc.description.abstractCa2 +/calmodulin-dependent protein kinase II (CaMKII) is activated by particulate matter (PM) isolated from ambient air and linked to prolonged repolarization and cardiac arrhythmia. We evaluated whether alpha B-crystallin (CryAB), a heat shock protein, could prevent the arrhythmogenic effects of PM by preventing CaMKII activation. CryAB was delivered into cardiac cells using a TAT-protein transduction domain (TAT-CryAB). ECGs were measured before and after tracheal exposure of diesel exhaust particles (DEP) and each intervention in adult Sprague–Dawley rats. After endotracheal exposure of DEP (200 μg/mL for 30 minutes, n = 11), QT intervals were prolonged from 115 ± 14 ms to 144 ± 20 ms (p = 0.03), and premature ventricular contractions were observed more frequently (0% vs. 44%) than control (n = 5) and TAT-Cry (n = 5). However, DEP-induced arrhythmia was not observed in TAT-CryAB (1 mg/kg) pretreated rats (n = 5). In optical mapping of Langendorff-perfused rat heats, compared with baseline, DEP infusion of 12.5 μg/mL (n = 12) increased apicobasal action potential duration (APD) differences from 2 ± 6 ms to 36 ± 15 ms (p < 0.001), APD restitution slope from 0.26 ± 0.07 to 1.19 ± 0.11 (p < 0.001) and ventricular tachycardia (VT) from 0% to 75% (p < 0.001). DEP infusion easily induced spatially discordant alternans. However, the effects of DEP were prevented by TAT-CryAB (1 mg/kg, n = 9). In rat myocytes, while DEP increased reactive oxygen species (ROS) generation and phosphated CaMKII, TAT-CryAB prevented these effects. In conclusion, CryAB, a small heat shock protein, might prevent the arrhythmogenic effects of PM by attenuating ROS generation and CaMKII activation.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfTOXICOLOGY AND APPLIED PHARMACOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAction Potentials-
dc.subject.MESHAir Pollutants/toxicity*-
dc.subject.MESHAnimals-
dc.subject.MESHArrhythmias, Cardiac/chemically induced-
dc.subject.MESHArrhythmias, Cardiac/prevention & control*-
dc.subject.MESHCalcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism-
dc.subject.MESHMale-
dc.subject.MESHMyocytes, Cardiac/metabolism-
dc.subject.MESHOxidative Stress/physiology*-
dc.subject.MESHParticulate Matter/toxicity*-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHReactive Oxygen Species/metabolism-
dc.subject.MESHVehicle Emissions/toxicity-
dc.subject.MESHVentricular Premature Complexes/chemically induced-
dc.subject.MESHVentricular Premature Complexes/prevention & control-
dc.subject.MESHalpha-Crystallin B Chain/administration & dosage-
dc.subject.MESHalpha-Crystallin B Chain/physiology*-
dc.titleAlpha B-crystallin prevents the arrhythmogenic effects of particulate matter isolated from ambient air by attenuating oxidative stress-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Preventive Medicine (예방의학)-
dc.contributor.googleauthorHyelim Park-
dc.contributor.googleauthorSanghoon Park-
dc.contributor.googleauthorHyunju Jeon-
dc.contributor.googleauthorByeong-Wook Song-
dc.contributor.googleauthorJin-Bae Kim-
dc.contributor.googleauthorChang-Soo Kim-
dc.contributor.googleauthorHui-Nam Pak-
dc.contributor.googleauthorKi-Chul Hwang-
dc.contributor.googleauthorMoon-Hyoung Lee-
dc.contributor.googleauthorJi Hyung Chung-
dc.contributor.googleauthorBoyoung Joung-
dc.identifier.doi10.1016/j.taap.2012.10.013-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03739-
dc.contributor.localIdA01042-
dc.contributor.localIdA04456-
dc.contributor.localIdA01760-
dc.contributor.localIdA01776-
dc.contributor.localIdA02026-
dc.contributor.localIdA02766-
dc.contributor.localIdA03562-
dc.contributor.localIdA03609-
dc.relation.journalcodeJ02742-
dc.identifier.eissn1096-0333-
dc.identifier.pmid23153557-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0041008X12004553-
dc.subject.keywordParticulate matter-
dc.subject.keywordHeat shock protein-
dc.subject.keywordAir pollution-
dc.subject.keywordArrhythmia-
dc.subject.keywordCa2 +/calmodulin-dependent protein kinase II-
dc.contributor.alternativeNameChung, Ji Hyung-
dc.contributor.alternativeNameKim, Chang Soo-
dc.contributor.alternativeNameHwang, Ki Chul-
dc.contributor.alternativeNamePark, Hye Lim-
dc.contributor.alternativeNamePak, Hui Nam-
dc.contributor.alternativeNameSong, Byeong Wook-
dc.contributor.alternativeNameLee, Moon Hyoung-
dc.contributor.alternativeNameJeon, Hyun Ju-
dc.contributor.alternativeNameJoung, Bo Young-
dc.contributor.affiliatedAuthorChung, Ji Hyung-
dc.contributor.affiliatedAuthorKim, Chang Soo-
dc.contributor.affiliatedAuthorHwang, Ki Chul-
dc.contributor.affiliatedAuthorPark, Hye Lim-
dc.contributor.affiliatedAuthorPak, Hui Nam-
dc.contributor.affiliatedAuthorSong, Byeong Wook-
dc.contributor.affiliatedAuthorLee, Moon Hyoung-
dc.contributor.affiliatedAuthorJeon, Hyun Ju-
dc.contributor.affiliatedAuthorJoung, Bo Young-
dc.rights.accessRightsnot free-
dc.citation.volume266-
dc.citation.number2-
dc.citation.startPage267-
dc.citation.endPage275-
dc.identifier.bibliographicCitationTOXICOLOGY AND APPLIED PHARMACOLOGY, Vol.266(2) : 267-275, 2013-
dc.identifier.rimsid28877-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Preventive Medicine (예방의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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