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Microfluidic flow fractionation device for label-free isolation of circulating tumor cells (CTCs) from breast cancer patients

Authors
 Kyung-A Hyun ; Kiho Kwon ; Hyunju Han ; Hyo-Il Jung ; Seung-Il Kim 
Citation
 Biosensors & Bioelectronics, Vol.40(1) : 206~212, 2013 
Journal Title
 Biosensors & Bioelectronics 
ISSN
 0956-5663 
Issue Date
2013
Abstract
Circulating tumor cells (CTCs) are dissociated from primary tumor and circulate in peripheral blood. They are regarded as the genesis of metastasis. Isolation and enumeration of CTCs serve as valuable tools for cancer prognosis and diagnosis. However, the rarity and heterogeneity of CTCs in blood makes it difficult to separate intact CTCs without loss. In this paper, we introduce a parallel multi-orifice flow fractionation (p-MOFF) device in which a series of contraction/expansion microchannels are placed parallel on a chip forming four identical channels. CTCs were continuously isolated from the whole blood of breast cancer patients by hydrodynamic forces and cell size differences. Blood samples from 24 breast cancer patients were analyzed (half were from metastatic breast cancer patients and the rest were from adjuvant breast cancer patients). The number of isolated CTCs varied from 0 to 21 in 7.5 ml of blood. Because our devices do not require any labeling processes (e.g., EpCAM antibody), heterogeneous CTCs can be isolated regardless of EpCAM expression.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/86133
DOI
10.1016/j.bios.2012.07.021
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Surgery
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Link
 http://www.sciencedirect.com/science/article/pii/S0956566312004617
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