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Microfluidic flow fractionation device for label-free isolation of circulating tumor cells (CTCs) from breast cancer patients

DC FieldValueLanguage
dc.contributor.author김승일-
dc.date.accessioned2014-12-18T08:20:42Z-
dc.date.available2014-12-18T08:20:42Z-
dc.date.issued2013-
dc.identifier.issn0956-5663-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/86133-
dc.description.abstractCirculating tumor cells (CTCs) are dissociated from primary tumor and circulate in peripheral blood. They are regarded as the genesis of metastasis. Isolation and enumeration of CTCs serve as valuable tools for cancer prognosis and diagnosis. However, the rarity and heterogeneity of CTCs in blood makes it difficult to separate intact CTCs without loss. In this paper, we introduce a parallel multi-orifice flow fractionation (p-MOFF) device in which a series of contraction/expansion microchannels are placed parallel on a chip forming four identical channels. CTCs were continuously isolated from the whole blood of breast cancer patients by hydrodynamic forces and cell size differences. Blood samples from 24 breast cancer patients were analyzed (half were from metastatic breast cancer patients and the rest were from adjuvant breast cancer patients). The number of isolated CTCs varied from 0 to 21 in 7.5 ml of blood. Because our devices do not require any labeling processes (e.g., EpCAM antibody), heterogeneous CTCs can be isolated regardless of EpCAM expression.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfBIOSENSORS & BIOELECTRONICS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBreast Neoplasms/pathology*-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Separation/instrumentation*-
dc.subject.MESHCell Separation/methods*-
dc.subject.MESHChemical Fractionation/instrumentation-
dc.subject.MESHEquipment Design-
dc.subject.MESHEquipment Failure Analysis-
dc.subject.MESHFlow Cytometry/instrumentation*-
dc.subject.MESHHumans-
dc.subject.MESHMicrofluidic Analytical Techniques/instrumentation*-
dc.subject.MESHNeoplastic Cells, Circulating/pathology*-
dc.titleMicrofluidic flow fractionation device for label-free isolation of circulating tumor cells (CTCs) from breast cancer patients-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorKyung-A Hyun-
dc.contributor.googleauthorKiho Kwon-
dc.contributor.googleauthorHyunju Han-
dc.contributor.googleauthorSeung-Il Kim-
dc.contributor.googleauthorHyo-Il Jung-
dc.identifier.doi10.1016/j.bios.2012.07.021-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00658-
dc.relation.journalcodeJ00330-
dc.identifier.eissn1873-4235-
dc.identifier.pmid22857995-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0956566312004617-
dc.subject.keywordCirculating tumor cell-
dc.subject.keywordMetastasis-
dc.subject.keywordFlow fractionation-
dc.subject.keywordBreast cancer-
dc.contributor.alternativeNameKim, Seung Il-
dc.contributor.affiliatedAuthorKim, Seung Il-
dc.rights.accessRightsnot free-
dc.citation.volume40-
dc.citation.number1-
dc.citation.startPage206-
dc.citation.endPage212-
dc.identifier.bibliographicCitationBIOSENSORS & BIOELECTRONICS, Vol.40(1) : 206-212, 2013-
dc.identifier.rimsid28803-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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