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SARS-CoV-2 spike-specific nasal-resident CD49a+CD8+ memory T cells exert immediate effector functions with enhanced IFN- production

Authors
 Min-Seok Rha  ;  Gyeongyeob Kim  ;  Sol Lee  ;  Jihye Kim  ;  Yeonsu Jeong  ;  Chan Min Jung  ;  Hae Eun Noh  ;  Ji Yun Noh  ;  Yong Min Kim  ;  Hyung-Ju Cho  ;  Chang-Hoon Kim  ;  Eui-Cheol Shin 
Citation
 NATURE COMMUNICATIONS, Vol.15(1) : 8355, 2024-09 
Journal Title
NATURE COMMUNICATIONS
Issue Date
2024-09
MeSH
Adult ; Antigens, CD / immunology ; Antigens, CD / metabolism ; CD8-Positive T-Lymphocytes* / immunology ; COVID-19 Vaccines / immunology ; COVID-19* / immunology ; COVID-19* / virology ; Female ; Humans ; Immunologic Memory / immunology ; Integrin alpha Chains ; Integrin alpha1 / immunology ; Integrin alpha1 / metabolism ; Interferon-gamma* / immunology ; Interferon-gamma* / metabolism ; Male ; Memory T Cells* / immunology ; Middle Aged ; Nasal Mucosa* / immunology ; Nasal Mucosa* / virology ; SARS-CoV-2* / immunology ; Spike Glycoprotein, Coronavirus* / immunology
Abstract
Virus-specific nasal resident T cells are important for protection against subsequent infection with a similar virus. Here we examine the phenotypes and functions of SARS-CoV-2-specific T cells in the nasal mucosa of vaccinated individuals with breakthrough infection (BTI) or without infection. Nasal tissues are obtained from participants during sinus surgery. Analysis of activation-induced markers implicates that a considerable proportion of spike (S)-reactive nasal CD8+ T cells express CD103, a tissue-resident marker. MHC-I multimer staining is performed to analyze the ex vivo phenotype and function of SARS-CoV-2 S-specific CD8+ T cells. We detect multimer+CD8+ T cells with tissue-resident phenotypes in nasal tissue samples from vaccinees without infection as well as vaccinees with BTI. Multimer+CD8+ T cells remain present in nasal tissues over one year after the last exposure to S antigen, although the frequency decreases. Upon direct ex vivo stimulation with epitope peptides, nasal multimer+CD8+ T cells-particularly the CD49a+ subset-exhibit immediate effector functions, including IFN-γ production. CITE-seq analysis of S-reactive AIM+CD8+ T cells confirms the enhanced effector function of the CD49a+ subset. These findings indicate that among individuals previously exposed to S antigen by vaccination or BTI, S-specific nasal-resident CD49a+CD8+ memory T cells can rapidly respond to SARS-CoV-2 during infection or reinfection.
Files in This Item:
T202406012.pdf Download
DOI
10.1038/s41467-024-52689-5
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Rha, Min-Seok(나민석) ORCID logo https://orcid.org/0000-0003-1426-7534
Cho, Hyung Ju(조형주) ORCID logo https://orcid.org/0000-0002-2851-3225
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/200848
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