Generation of a human induced pluripotent stem cell line (YUCMi020-A) from peripheral blood mononuclear cells derived from a female with the Jr(a-) blood type

Youn Keong Cho; Hyun-Kyung Kim; Eun Jung Suh; Hyun Ok Kim; Sinyoung Kim

doi:10.1016/j.scr.2024.103434

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Generation of a human induced pluripotent stem cell line (YUCMi020-A) from peripheral blood mononuclear cells derived from a female with the Jr(a-) blood type

Authors: Youn Keong Cho ; Hyun-Kyung Kim ; Eun Jung Suh ; Hyun Ok Kim ; Sinyoung Kim

Citation: STEM CELL RESEARCH, Vol.77 : 103434, 2024-06

Journal Title: STEM CELL RESEARCH

ISSN: 1873-5061

Issue Date: 2024-06

MeSH: ATP Binding Cassette Transporter, Subfamily G, Member 2 / genetics ; ATP Binding Cassette Transporter, Subfamily G, Member 2 / metabolism ; Blood Group Antigens / metabolism ; Cell Differentiation ; Cell Line ; Female ; Humans ; Induced Pluripotent Stem Cells* / cytology ; Induced Pluripotent Stem Cells* / metabolism ; Leukocytes, Mononuclear* / cytology ; Leukocytes, Mononuclear* / metabolism

Abstract: The Jra antigen, the only antigen within the JR blood group system, is a high-prevalence red blood cell (RBC) antigen found in over 99 % of the global population. An induced pluripotent stem cell line (YUCMi020-A) was generated from peripheral blood drawn from a Jr(a−) phenotype individual, who was homozygous for a null mutation of ABCG2*01N.01 (rs72552713, c.376C>T; p.Gln126*). The generated line exhibited pluripotent characteristics and no chromosomal aberrations. This cell line will serve as a cell source, enabling us to produce RBCs with the Jr(a−) phenotype in vitro, which can be used for transfusing individuals with anti-Jra antibodies. © 2024