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Applicability of the cobas 6800 System for Epstein-Barr viral load quantitation using whole-blood specimens

Authors
 Junhyup Song  ;  Sinyoung Kim  ;  Eunmin Kwak  ;  Younhee Park 
Citation
 AMERICAN JOURNAL OF CLINICAL PATHOLOGY, Vol.161(3) : 273-282, 2024-03 
Journal Title
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
ISSN
 0002-9173 
Issue Date
2024-03
MeSH
Cross Reactions ; Herpesvirus 4, Human* / genetics ; Humans ; Plasma* ; Viral Load
Keywords
Epstein-Barr virus ; analytical performance ; cobas 6800 ; methods comparison ; whole blood
Abstract
Objectives: This study aimed to evaluate the analytical performance of the cobas 6800 System (Roche Diagnostics) and assess the feasibility of using whole-blood specimens instead of plasma.



Methods: The analytical performance of the cobas EBV test (Roche Diagnostics) was evaluated. Thereafter, 120 clinical samples were collected to compare the cobas EBV test and the artus EBV RG PCR Kit (Qiagen). The results of the cobas EBV test conducted using paired plasma as well as 5× and 10× diluted whole-blood specimens were compared with those of the artus EBV RG PCR Kit performed using whole blood.



Results: The precision of the cobas EBV test was acceptable, and its linearity was confirmed to be within the range of 2.85 to 6.89 log IU/mL. Cross-reactivity was not observed. The best qualitative agreement (Cohen κ = 0.733) was observed using 5× diluted whole blood; the best quantitative correlation (Spearman correlation coefficient = 0.6865) was observed using 10× diluted whole blood.



Conclusions: A significant discrepancy was observed in the results obtained from the 2 assays because of the different specimens used. We observed, however, that diluting whole blood before conducting the cobas EBV test effectively resolved polymerase chain reaction inhibition and viscosity issues, leading to an acceptable correlation with the results from the artus EBV RG PCR Kit conducted using whole blood.
Full Text
https://academic.oup.com/ajcp/article-lookup/doi/10.1093/ajcp/aqad146
DOI
10.1093/ajcp/aqad146
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Park, Youn Hee(박윤희) ORCID logo https://orcid.org/0000-0001-8458-1495
Song, Junhyup(송준협)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/198802
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