37 72

Cited 0 times in

Circulating Tumor DNA Reflects Histologic and Clinical Characteristics of Various Lymphoma Subtypes

Authors
 Jin Ju Kim  ;  Hye Min Kim  ;  Hongkyung Kim  ;  Soo-Jeong Kim  ;  Seung-Tae Lee  ;  Jong Rak Choi  ;  Saeam Shin  ;  Doh Yu Hwang 
Citation
 CANCER RESEARCH AND TREATMENT, Vol.56(1) : 314-323, 2024-01 
Journal Title
CANCER RESEARCH AND TREATMENT
ISSN
 1598-2998 
Issue Date
2024-01
MeSH
Biomarkers, Tumor / genetics ; Circulating Tumor DNA* / genetics ; High-Throughput Nucleotide Sequencing ; Humans ; Liquid Biopsy ; Lymphoma* ; Mutation
Keywords
Circulating tumor DNA ; Liquid biopsy ; Lymphoma
Abstract
Purpose We designed and evaluated the clinical performance of a plasma circulating tumor DNA (ctDNA) panel of 112 genes in various subtypes of lymphoma.Materials and Methods Targeted deep sequencing with an error-corrected algorithm was performed in ctDNA from plasma samples that were collected before treatment in 42 lymphoma patients. Blood buffy coat was utilized as a germline control. We evaluated the targeted gene panel using mutation detection concordance on the plasma samples with matched tissue samples analyzed the mutation profiles of the ctDNA.Results Next-generation sequencing analysis using matched tissue samples was available for 18 of the 42 patients. At least one mutation was detected in the majority of matched tissue biopsy samples (88.9%) and plasma samples (83.3%). A considerable number of mutations (40.4%) that were detected in the tissue samples were also found in the matched plasma samples. Majority of patients (21/42) were diffuse large B cell lymphoma patients. The overall detection rate of ctDNA in patients was 85.7% (36/42). The frequently mutated genes included PIM1, TET2, BCL2, KMT2D, KLHL6, HIST1H1E, and IRF8. A cutoff concentration (4,506 pg/mL) of ctDNA provided 88.9% sensitivity and 82.1% specificity to predict ctDNA mutation detection. The ctDNA concentration correlated with elevated lactate dehydrogenase level and the disease stage.Conclusion Our design panel can detect many actionable gene mutations, including those at low frequency. Therefore, liquid biopsy can be applied clinically in the evaluation of lymphoma patients, especially in aggressive lymphoma patients.
Files in This Item:
T202400624.pdf Download
DOI
10.4143/crt.2023.667
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Soo Jeong(김수정) ORCID logo https://orcid.org/0000-0001-8859-3573
Kim, Jin Ju(김진주) ORCID logo https://orcid.org/0000-0001-9166-1848
Kim, Hye Min(김혜민) ORCID logo https://orcid.org/0000-0002-2899-9480
Shin, Saeam(신새암) ORCID logo https://orcid.org/0000-0003-1501-3923
Lee, Seung-Tae(이승태) ORCID logo https://orcid.org/0000-0003-1047-1415
Choi, Jong Rak(최종락) ORCID logo https://orcid.org/0000-0002-0608-2989
Hwang, Doh Yu(황도유) ORCID logo https://orcid.org/0000-0002-0750-0011
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/198559
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links