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Saliva-based Proteinase K method: A rapid and reliable diagnostic tool for the detection of SARS-COV-2 in children

Authors
 Min Jung Kim  ;  Pil-Gu Park  ;  Su Jin Hwang  ;  Seung Jun Bang  ;  Jae Hwa Jung  ;  Eun Bin Kown  ;  Eun Kyung Sul  ;  Kyung Chul Song  ;  Joon-Sik Choi  ;  Kyeo Re Han  ;  Hye Young Lee  ;  Dong Hyun Kim  ;  Jae Myun Lee 
Citation
 JOURNAL OF MEDICAL VIROLOGY, Vol.96(1) : e29361, 2024-01 
Journal Title
JOURNAL OF MEDICAL VIROLOGY
ISSN
 0146-6615 
Issue Date
2024-01
MeSH
COVID-19 Testing ; COVID-19* / diagnosis ; Child ; Child, Preschool ; Endopeptidase K ; Humans ; Nasopharynx ; RNA ; SARS-CoV-2 / genetics ; Saliva* ; Specimen Handling
Keywords
COVID-19 ; Proteinase K ; SARS-COV-2 ; children ; diagnostics ; saliva
Abstract
Early and accurate detection of viruses in children might help prevent transmission and severe diseases. In this study, the severe acute respiratory syndrome coronavirus 2 (SARS‐COV‐2) detection in children was evaluated using saliva specimens with a Proteinase K (PTK)‐based RNA preparation, as saliva collection is a simple and noninvasive procedure, even in young children, with fewer concerns about sample contamination. The saliva‐based PTK and the conventional paired nasopharyngeal aspiration (NPA)‐based detection methods were compared between COVID‐19‐positive and ‐negative children. In addition, the detection rate for SARS‐COV‐2 and the difference between admission and discharge by the saliva‐based PTK method was tested in COVID‐19 patients. The diagnostic accuracy of the saliva‐based PTK method was 98.8% compared to NP swab‐based reverse transcriptase polymerase chain reaction. Saliva samples showed high sensitivity (94.1%) and specificity (100%) when using the PTK method. Furthermore, the saliva‐based PTK method significantly reduced the test processing time by 2 h. Notably, Ct values at discharge increased in saliva samples compared with those at admission, which might indicate patients' clinical conditions or virus activity. In conclusion, the saliva‐based PTK implemented in this study streamlines RNA extraction, making the process faster, safer, and more cost‐effective, demonstrating that this method is a rapid and reliable diagnostic tool for SARS‐CoV‐2 detection in children.
Full Text
https://onlinelibrary.wiley.com/doi/10.1002/jmv.29361
DOI
10.1002/jmv.29361
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Min Jung(김민정) ORCID logo https://orcid.org/0000-0002-5634-9709
Song, Kyungchul(송경철) ORCID logo https://orcid.org/0000-0002-8497-5934
Lee, Jae Myun(이재면) ORCID logo https://orcid.org/0000-0002-5273-3113
Jung, Jae Hwa(정재화)
Choi, Joon Sik(최준식) ORCID logo https://orcid.org/0000-0002-5587-2960
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/198539
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