Antibodies, Bispecific / pharmacology ; Antigens, CD20 ; Cell Death ; Humans ; Lymphoma, B-Cell* / drug therapy ; Receptors, Tumor Necrosis Factor, Type I / therapeutic use ; Tumor Necrosis Factor-alpha*
Keywords
Antibody binding-induced cell death ; BNHL (B cell non-Hodgkin’s lymphoma) ; Bispecific or fusion proteins ; Obinutuzumab ; TNFR1
Abstract
Obinutuzumab is a therapeutic antibody for B cell non-Hodgkin’s Lymphoma (BNHL), which is a glyco-engineered anti-CD20 antibody with enhanced antibody-dependent cellular cytotoxicity (ADCC) and causes binding-induced direct cell death (DCD) through lysosome membrane permeabilization (LMP). Tumour necrosis factor receptor 1 (TNFR1), a pro-inflammatory death receptor, also evokes cell death, partly through lysosomal rupture. As both obinutuzumab- and TNFR1-induced cell deaths are mediated by LMP and combining TNFR1 and obinutuzumab can amplify LMP-mediated cell death, we made dual-targeting antibody for CD20 and TNFR1 to enhance DCD of obinutuzumab. Obinutuzumab treatment-induced CD20 and TNFR1 colocalisation, and TNFR1-overexpressing cells showed increased obinutuzumab-induced DCD. Two targeting modes, anti-CD20/TNFR1 bispecific antibodies (bsAbs), and obinutuzumab-TNFα fusion proteins (OBI-TNFα