34 118

Cited 0 times in

European Committee on Antimicrobial Susceptibility Testing-Recommended Rapid Antimicrobial Susceptibility Testing of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus From Positive Blood Culture Bottles

 Jong-Min Park  ;  Mijung Kwon  ;  Ki Ho Hong  ;  Hyukmin Lee  ;  Dongeun Yong 
 ANNALS OF LABORATORY MEDICINE, Vol.43(5) : 443-450, 2023-09 
Journal Title
Issue Date
Anti-Bacterial Agents / pharmacology ; Anti-Infective Agents* ; Blood Culture ; Escherichia coli ; Humans ; Klebsiella pneumoniae ; Microbial Sensitivity Tests ; Staphylococcal Infections* ; Staphylococcus aureus
Categorical agreement ; Escherichia coli ; European Committee on Antimicrobial Susceptibility Testing ; Klebsiella pneumoniae ; Microbial sensitivity test ; Rapid antimicrobial susceptibility testing ; Sepsis ; Staphylococcus aureus ; United States Food and Drug Administration
Background: Early diagnosis and treatment are important for a good prognosis of bloodstream infections. The European Committee on Antimicrobial Susceptibility Testing (EUCAST) recommends rapid antimicrobial susceptibility testing (RAST) based on the disk diffusion methodology for 4, 6, and 8 hours of incubation. We evaluated EUCAST-RAST of Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus from positive blood culture bottles.

Methods: Twenty strains of E. coli, K. pneumoniae, and S. aureus were tested using EUCAST-RAST. Ten antimicrobial agents against E. coli and K. pneumoniae and four agents against S. aureus were tested. The diameter of the inhibition zone (mm) was compared with the minimal inhibitory concentration (μg/mL) obtained using the Sensititre AST system (TREK Diagnostic Systems, East Grinstead, UK).

Results: For E. coli, the percentage of total categorical agreement (CA) was 69.5% at 4 hours, and 87% at 8 hours. For K. pneumoniae, the total CA was 89% at 4 hours, and 95.5% at 6 hours. For S. aureus, the total CA was 100% after 4 hours. Discrepancies were observed mainly for E. coli with β-lactam antimicrobial agents, and the numbers of errors decreased over time.

Conclusions: EUCAST-RAST for K. pneumoniae and S. aureus met the United States Food and Drug Administration criteria at 6 and 4 hours, respectively, whereas that for E. coli did not meet the criteria for up to 8 hours. RAST can shorten the turn-around testing time by more than one day; therefore, if applied accurately according to laboratory conditions, antimicrobial agent results can be reported faster.
Files in This Item:
T202304166.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Hyuk Min(이혁민) ORCID logo https://orcid.org/0000-0002-8523-4126
Hong, Ki Ho(홍기호) ORCID logo https://orcid.org/0000-0002-5700-9036
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.